%0 Journal Article
	%A Hanife Genc
	%D 2015
	%J International Journal of Agricultural and Biosystems Engineering
	%B World Academy of Science, Engineering and Technology
	%I Open Science Index 104, 2015
	%T Sex Differentiation of Elm Nymphalid (Nymphalis polychloros Linnaeus, 1758) on Pupal Stage
	%U https://publications.waset.org/pdf/10002062
	%V 104
	%X This study was conducted to determine sex
differentiation of laboratory reared Elm nymphalid (Nymphalis
polychloros Linnaeus, 1758) by examining the morphological
structure of pupal stage. Laboratory colony of elm nymphalid, reared
on pear leaves, was used to set up experiments. It was performed
with 5 replications having 8 pupae for each replication. Dorsal,
ventral and lateral parts of external morphological structures of pupae
were examined by Olympus SZX9 stereozoom microscope and
photographed. When fully grown, mature larvae wander the highest
part of the rearing cage and pupae were formed hanging by
cremaster. After completing prepupa stage about 1.5±0.3 days, they
all pupated. Pupal stage was completed at 24±1°C about 4.38±1.20
days. Pupal weights were 0.483±0.05 g in females and 0.392±0.08 g
(n=40) in males respectively. Pupal emergence rate was 95%, with
22 females and 16 males. Examinations of ventral parts of 8th, 9th,
and 10th abdominal segments revealed that anal opening were found
at 10th abdominal segment in both sexes, 3 lumps were determined at
9th abdominal segments then the specific opening structure at 8th
segment was only found on female pupae.
	%P 899 - 902