%0 Journal Article
	%A C.-C. Lin and  S.-C. Kan and  C.-W. Yeh and  C.-I Chen and  C.-J. Shieh and  Y.-C. Liu
	%D 2015
	%J International Journal of Biotechnology and Bioengineering
	%B World Academy of Science, Engineering and Technology
	%I Open Science Index 103, 2015
	%T Kinetics Study for the Recombinant Cellulosome to the Degradation of Chlorella Cell Residuals
	%U https://publications.waset.org/pdf/10001986
	%V 103
	%X In this study, lipid-deprived residuals of microalgae
were hydrolyzed for the production of reducing sugars by using the
recombinant Bacillus cellulosome, carrying eight genes from the
Clostridium thermocellum ATCC27405. The obtained cellulosome
was found to exist mostly in the broth supernatant with a cellulosome
activity of 2.4 U/mL. Furthermore, the Michaelis-Menten constant
(Km) and Vmax of cellulosome were found to be 14.832 g/L and 3.522
U/mL. The activation energy of the cellulosome to hydrolyze
microalgae LDRs was calculated as 32.804 kJ/mol.
	%P 782 - 785