@article{(Open Science Index):https://publications.waset.org/pdf/10001685,
	  title     = {Gold-Mediated Modification of Apoferritin Surface with Targeting Antibodies},
	  author    = {Simona Dostalova and  Pavel Kopel and  Marketa Vaculovicova and  Vojtech Adam and  Rene Kizek},
	  country	= {},
	  institution	= {},
	  abstract     = {To ensure targeting of apoferritin nanocarrier with
encapsulated doxorubicin drug, we used a peptide linker based on a
protein G with N-terminus affinity towards Fc region of antibodies.
To connect the peptide to the surface of apoferritin, the C-terminus of
peptide was made of cysteine with affinity to gold. The surface of
apoferritin with encapsulated doxorubicin (APODOX) was coated
either with gold nanoparticles (APODOX-Nano) or gold(III) chloride
hydrate reduced with sodium borohydride (APODOX-HAu). The
reduction with sodium borohydride caused a loss of doxorubicin
fluorescent properties and probably accompanied with the loss of its
biological activity. Fluorescent properties of APODOX-Nano were
similar to the unmodified APODOX; therefore it was more suited for
the intended use. To evaluate the specificity of apoferritin modified
with antibodies, ELISA-like method was used with the surface of
microtitration plate wells coated by the antigen (goat anti-human IgG
antibodies). To these wells, the nanocarrier was applied. APODOX
without the modification showed 5× lower affinity to the antigen than
APODOX-Nano modified gold and targeting antibodies (human IgG
antibodies).},
	    journal   = {International Journal of Medical and Health Sciences},
	  volume    = {9},
	  number    = {7},
	  year      = {2015},
	  pages     = {550 - 553},
	  ee        = {https://publications.waset.org/pdf/10001685},
	  url   	= {https://publications.waset.org/vol/103},
	  bibsource = {https://publications.waset.org/},
	  issn  	= {eISSN: 1307-6892},
	  publisher = {World Academy of Science, Engineering and Technology},
	  index 	= {Open Science Index 103, 2015},
	}