{"title":"Large Scale Production of Polyhydroxyalkanoates (PHAs) from Wastewater: A Study of Techno-Economics, Energy Use and Greenhouse Gas Emissions","authors":"Cora Fernandez Dacosta, John A. Posada, Andrea Ramirez","volume":101,"journal":"International Journal of Mechanical and Industrial Engineering","pagesStart":450,"pagesEnd":456,"ISSN":"1307-6892","URL":"https:\/\/publications.waset.org\/pdf\/10001172","abstract":"
The biodegradable family of polymers
\r\npolyhydroxyalkanoates is an interesting substitute for convectional
\r\nfossil-based plastics. However, the manufacturing and environmental
\r\nimpacts associated with their production via intracellular bacterial
\r\nfermentation are strongly dependent on the raw material used and on
\r\nenergy consumption during the extraction process, limiting their
\r\npotential for commercialization. Industrial wastewater is studied in
\r\nthis paper as a promising alternative feedstock for waste valorization.
\r\nBased on results from laboratory and pilot-scale experiments, a
\r\nconceptual process design, techno-economic analysis and life cycle
\r\nassessment are developed for the large-scale production of the most
\r\ncommon type of polyhydroxyalkanoate, polyhydroxbutyrate.
\r\nIntracellular polyhydroxybutyrate is obtained via fermentation of
\r\nmicrobial community present in industrial wastewater and the
\r\ndownstream processing is based on chemical digestion with
\r\nsurfactant and hypochlorite. The economic potential and
\r\nenvironmental performance results help identifying bottlenecks and
\r\nbest opportunities to scale-up the process prior to industrial
\r\nimplementation. The outcome of this research indicates that the
\r\nfermentation of wastewater towards PHB presents advantages
\r\ncompared to traditional PHAs production from sugars because the
\r\nnull environmental burdens and financial costs of the raw material in
\r\nthe bioplastic production process. Nevertheless, process optimization
\r\nis still required to compete with the petrochemicals counterparts.<\/p>\r\n","references":"[1] M. Carus, \u201cProduction capacities for bio-based polymers in Europe -\r\nstatus quo and trends towards 2020,\u201d Nova-Institute for Ecology and\r\nInnovation. Press release. July 2013.\r\n[2] R. J. Van Wegen, Y. Ling, and A. P. J Middelberg, \u201cIndustrial\r\nproduction of polyhydroxyalkanoates using Escherichia coli: An\r\neconomic analysis,\u201d Chemical Engineering Research and Design, vol.\r\n76, pp. 417-426, March 1998.\r\n[3] C. S. K., Reddy, R. Ghai, Rashmi, and V. C. Kalia,\r\n\u201cPolyhydroxyalkanoates: an overview,\u201d Bioresource Technology, vol.\r\n87, pp. 137-146, April 2003.\r\n[4] R. A. J Verlinden, D. J. Hill, M. A. Kenward, C. D. Williams, and I.\r\nRadecka, \u201cBacterial synthesis of biodegradable polyhydroxyalkanoates,\u201d\r\nJournal of Applied Microbiology, vol. 102, pp. 1437\u20131449, June 2007.\r\n[5] L. L. Madison, and G.W. Huisman, \u201cMetabolic engineering of poly(3-\r\nhydroxyalkanoates): from DNA to plastic,\u201d Microbiology and Molecular\r\nBiology Reviews, vol. 63, pp. 21-53, March 1999.\r\n[6] H. Salehizadeh, and M. C. M. Van Loosdrecht, \u201cProduction of\r\npolyhydroxyalkanoates by mixed culture: recent trends and\r\nbiotechnological importance,\u201d Biotechnology Advances, vol. 22, pp.\r\n261-279, January 2004.\r\n[7] Y. Jiang, L. Marang, J. Tamis, M. C. M. van Loosdrecht, H. Dijkman,\r\nand R. Kleerebezem, \u201cWaste to resource: Converting paper mill\r\nwastewater to bioplastic,\u201d Water Research, vol. 46, pp. 5517-5530,\r\nNovember 2012.\r\n[8] J. Tamis, L. Katlin, Y. Jiang, M. C. M. van Loosdrecht, and R.\r\nKleerebezem, \u201cEnrichment of Plasticicumulans acidivorans at pilot-scale\r\nfor PHA production on industrial wastewater,\u201d Journal of\r\nBiotechnology, to be published.\r\n[9] N. Jacquel, C.-W. Lo, Y.-H. Wei, H.-S. Wu, and S. S. Wang, \u201cIsolation\r\nand purification of bacterial poly(3-hydroxyalkanoates),\u201d Biochemical\r\nEngineering Journal, vol. 39, pp. 15\u201327, April 2008.\r\n[10] L. A. Smith, K. J. Roberts, D. Machin, and G. McLeod, \u201cAn\r\nexamination of the solution phase and nucleation properties of sodium,\r\npotassium and rubidium dodecyl sulphates,\u201d Journal of Crystal Growth,\r\nvol. 226, pp. 158\u2013167, June 2001.\r\n[11] R. Smith, Chemical process design and integration, pp. 17-31,\r\nChichester: John Wiley & Sons, 2005.\r\n[12] R. K. Sinnott, Coulson & Richardson's chemical engineering. Volume 6:\r\nchemical engineering design, fourth ed., pp. 246-279, Oxford: Elsevier,\r\n2005.\r\n[13] G. D. Ulrich, and P. T. Vasudevan, \u201cHow to estimate utility costs,\u201d\r\nChemical Engineering, vol. 4, pp. 66-69, April 2006.\r\n[14] International Standards Organization (ISO), ISO 14040:2006,\r\n\u201cEnvironmental management. Life cycle assessment. Principles and\r\nframework,\u201d 2006a.\r\n[15] International Standards Organization (ISO), ISO 14044:2006,\r\n\u201cEnvironmental management. Life cycle assessment. Requirements and\r\nguidelines,\u201d 2006b.\r\n[16] U.S. Environmental Protection Agency, \u201cAccounting framework for\r\nbiogenic CO2 emissions from stationary sources,\u201d Office of\r\nAtmospheric Programs, Climate Change Division, Washington, DC,\r\n2011.\r\n[17] P. Pawelzik, M. Carus, J. Hotchkiss, R. Narayan, S. Selke, M. Wellisch,\r\nM. Weiss, B. Wicke, and M. K. Patel, \u201cCritical aspects in the life cycle\r\nassessment (LCA) of bio-based materials\u2013Reviewing methodologies and\r\nderiving recommendations,\u201d Resources, Conservation and Recycling,\r\nvol. 73, pp. 211\u2013228, April 2013.\r\n[18] Y. Jiang, G. Mikova, R. Kleerebezem, L. A. M. van der Wielen, and\r\nM.C. Cuellar, \u201cFeasibility study of an alkaline-based chemical treatment\r\nfor polyhydroxyalkanoates purification from mixed enriched culture,\u201d\r\nunpublished.\r\n[19] D. Michael, \u201cBiodegradable polymer supply chains: implications and\r\nopportunities for agriculture,\u201d Wondu Holdings, Sydney, 2004.\r\n[20] J. Choi, and S. Y. Lee, \u201cProcess analysis and economic evaluation for\r\nPoly(3-hydroxybutyrate) production by fermentation,\u201d Bioprocess\r\nEngineering, vol. 17, pp. 335-342, November 1997.\r\n[21] M. Akiyama, T. Tsugea, and Y. Doia, \u201cEnvironmental life cycle\r\ncomparison of polyhydroxyalkanoates produced from renewable carbon\r\nresources by bacterial fermentation,\u201d Polymer Degradation and Stability,\r\nvol. 80, pp. 183\u2013194, February 2003.\r\n[22] S. Y. Lee, and J. Choi, \u201cEffect of fermentation performance on the\r\neconomics of poly(3-hydroxybutyrate) production by Alcaligenes latus,\u201d\r\nPolymer Degradation and Stability, vol. 59, pp. 387-393, January 1998.\r\n[23] J. A. Posada, J. M. Naranjo, J. A. L\u00f3pez, J. C. Higuita, and C. A.\r\nCardona, \u201cDesign and analysis of poly-3-hydroxybutyrate production\r\nprocesses from crude glycerol,\u201d Process Biochemistry, vol. 46, pp. 310\u2013\r\n317, January 2011.\r\n[24] N. Gurieff, and P. Lant, \u201cComparative life cycle assessment and\r\nfinancial analysis of mixed culture polyhydroxyalkanoate production,\u201d\r\nBioresource Technology, vol. 98, pp. 3393\u20133403, July 2007.\r\n[25] J. M. Naranjo, J. A. Posada, J. C. Higuita, and C. A Cardona,\r\n\u201cValorization of glycerol through the production of biopolymers: The\r\nPHB case using Bacillus megaterium,\u201d Bioresource Technology, vol.\r\n133, pp.38\u201344, April 2013.\r\n[26] T. Mumtaz, N. A. Yahaya, S.Abd-Aziz, N. A. Rahman, P. L. Yee, Y.\r\nShirai, and M. A. Hassan, \u201cTurning waste to wealth-biodegradable\r\nplastics polyhydroxyalkanoates from palm oil mill effluent - a Malaysian\r\nperspective,\u201d Journal of Cleaner Production, vol. 18, pp. 393-1402, June\r\n2010.\r\n[27] ICIS Pricing. http:\/\/www.icis.com\/\r\n[28] M. Patel, C. Bastioli, L. Marini, and E. W\u00fcrdinger, \u201cLife cycle\r\nassessment of bio-based polymers and natural fibres composites,\u201d\r\nBiopolymers online, vol.10, January 2005.\r\n[29] R. Briones, L. Tai, E. Daoud, and J. Hart, \u201cLife Cycle Assessment.\r\nIdentifying process improvement opportunities and assessing\r\nalternatives,\u201d California environmental protection agency department of\r\ntoxic substances control, DTSC, Plastics Hazards Reduction Unit, June\r\n2011.\r\n[30] A. Liebich, and J. Giegrich, \u201cEco-profiles of the European plastics\r\nindustry. Polyethylene terephthalate (PET). Bottle grade,\u201d Plastics\r\nEurope, Heidelberg, April 2010.","publisher":"World Academy of Science, Engineering and Technology","index":"Open Science Index 101, 2015"}