{"title":"Modified Poly(pyrrole) Film Based Biosensors for Phenol Detection","authors":"S. Korkut, M. S. Kilic, E. Erhan","volume":99,"journal":"International Journal of Environmental and Ecological Engineering","pagesStart":439,"pagesEnd":443,"ISSN":"1307-6892","URL":"https:\/\/publications.waset.org\/pdf\/10000759","abstract":"
In order to detect and quantify the phenolic contents
\r\nof a wastewater with biosensors, two working electrodes based on
\r\nmodified Poly(Pyrrole) films were fabricated. Enzyme horseradish
\r\nperoxidase was used as biomolecule of the prepared electrodes.
\r\nVarious phenolics were tested at the biosensor. Phenol detection was
\r\nrealized by electrochemical reduction of quinones produced by
\r\nenzymatic activity. Analytical parameters were calculated and the
\r\nresults were compared with each other.<\/p>\r\n","references":"[1] T. Ruzgas, E. Cs\u00f6regi, J. Emneus, L. Gorton, G. Makro-Varga,\r\n\u201cPeroxidase-modified electrodes: Fundamentals and application,\u201d Anal.\r\nChim. Acta, vol. 330, pp. 123-138, September 1996.\r\n[2] J. Yu, S. Liu, H. Ju, \u201cMediator-free phenol sensor based on titania solgel\r\nencapsulation matrix for immobilization of tyrosinase by a vapor\r\ndeposition method,\u201d Biosens. Bioelectron., vol. 19, pp. 509-514,\r\nDecember 2003.\r\n[3] C. Capannesi, I. Ilaria Palchetti, M. Macsini, A. Parenti,\r\n\u201cElectrochemical sensor and biosensor for polyphenols detection in\r\nolive oils,\u201d Food Chemistry, vol. 71, pp. 553-562, December 2000.\r\n[4] J. Metzger, M. Reiss, W. Hartmeier, \u201cAmperometric phenol biosensor\r\nbased on a thermostable phenol hydroxylase,\u201d Biosens. Bioelectron., vol.\r\n13, pp. 1077-1082, November 1998.\r\n[5] S. Rodriguez-Mozaz, M. J. Lopez de Alda, M. P. Marco, D. Barcelo,\r\n\u201cBiosensors for environmental monitoring,\u201d Talanta, vol. 65, pp. 291-\r\n297, January 2005.\r\n[6] Y. L. Zhaou, R. H. Tian, J. F. Zhi, \u201cAmperometric biosensor based on\r\ntyrosinase immoblized on a boron-doped diamond electrode,\u201d Biosens.\r\nBioelectron., vol. 22, pp. 822-828, January 2007.\r\n[7] S. C. Chang, K. Rawson, C. J. McNeil, \u201cDisposable tyrosinaseperoxidase\r\nbi-enzyme sensor for amperometric detection of phenols,\u201d\r\nBiosens. Bioelectron., vol. 17, pp. 1015-1023, December 2002.\r\n[8] A. Lindgren, J. Emn\u00b4eus, T. Ruzgas, L. Gorton, G. Marko- Varga,\r\n\u201cAmperometric detection of phenols using peroxidase-modified graphite\r\nelectrodes,\u201d Anal. Chim. Acta, vol. 347, pp. 51-62, July 1997.\r\n[9] Y. C. Tsai, C. Cheng-Chiu, \u201cAmperometric biosensors based on\r\nmultiwalled carbon nanotube-Nafion-tyrosinase nanobiocomposites for\r\nthe determination of phenolic compounds,\u201d Sens. Actuators B: Chem.,\r\nvol. 125, pp. 10-16, July 2007.\r\n[10] Q. Fan, D. Shan, H. Xue, Y. He, S. Cosnier, \u201cAmperometric phenol\r\nbiosensor based on laponite-clay chitosan nanocomposite matrix,\u201d\r\nBiosens. Bioelectron., vol. 22, pp. 816-821, January 2007.\r\n[11] Rajesh, K. Kaneto, \u201cA new tyrosinase biosensor based on covalent\r\nimmobilization of enzyme on N-(3-aminopropyl) pyrrole polymer film,\u201d\r\nCurr. Appl. Phys., vol. 5, pp. 178-183, February 2005.\r\n[12] S. Korkut Ozoner, M. Yalvac Can, B. Keskinler, E. Erhan, \u201cA novel\r\nPoly(gluteraldehyde-co-pyrrole)\/horseradish peroxidase composite film\r\nelectrode,\u201d Anal. Lett., vol. 42, pp. 3058-3072, November 2009.\r\n[13] S. Korkut Ozoner, \u201cPoly(glycidyl methacrylate-co-3-thienylmethyl\r\nmethacrylate based working electrodes for hydrogenperoxide\r\nbiosensing,\u201d J Chem. Technol. Biotechnol., vol. 87, pp. 146-152,\r\nJanuary 2012.","publisher":"World Academy of Science, Engineering and Technology","index":"Open Science Index 99, 2015"}