C. Nobre and C. C. Castro and A.-L. Hantson and J. A. Teixeira and L. R. Rodrigues and G. De Weireld
Production of HighContent FructoOligosaccharides
158 - 163
2015
9
2
International Journal of Nutrition and Food Engineering
https://publications.waset.org/pdf/10000470
https://publications.waset.org/vol/98
World Academy of Science, Engineering and Technology
Fructooligosaccharides (FOS) are produced from
sucrose by Aureobasidium pullulans in yields between 4060
(ww). To increase the amount of FOS it is necessary to remove the
small, nonprebiotic sugars, present. Two methods for producing
highpurity FOS have been developed the use of microorganisms
able to consume small saccharides; and the use of continuous
chromatography to separate sugars simulated moving bed (SMB). It
is herein proposed the combination of both methods. The aim of this
study is to optimize the composition of the fermentative broth (in
terms of salts and sugars) that will be further purified by SMB. A
yield of 0.63 gFOS.gSucrose1 was obtained with A. pullulans using low
amounts of salts in the initial fermentative broth. By removing the
small sugars, Saccharomyces cerevisiae and Zymomonas mobilis
increased the percentage of FOS from around 56.0 to 83 (ww) in
average, losing only 10 (ww) of FOS during the recovery process.
Open Science Index 98, 2015