The Effect of Different Levels of Seed and Extract of Harmal (Peganum harmala L.) on Immune Responses of Broiler Chicks
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The Effect of Different Levels of Seed and Extract of Harmal (Peganum harmala L.) on Immune Responses of Broiler Chicks

Authors: M. Toghyani, A. Ghasemi, S. A. Tabeidian

Abstract:

The present study was carried out to evaluate the effect of different levels of dietary seed and extract of Harmal (Peganum harmala L.) on immunity of broiler chicks. A total of 350 one-day old broiler chicks (Ross 308) were randomly allocated to five dietary treatments with four replicates pen of 14 birds each. Dietary treatments consisted of control, 1 and 2 g/kg Harmal seed in diet, 100 and 200 mg/L Harmal seed extract in water. Broilers received dietary treatments from 1 to 42 d. Two birds from each pen were randomly weighed and sacrificed at 42 d of age, the relative weight of lymphoid organs (bursa of Fabercius and spleen) to live weight were calculated. Antibody titers against Newcastle and influenza viruses and sheep red blood cell were measured at 30 d of age. Results showed that the relative weights of lymphoid organs were not affected by dietary treatments. Furthermore, antibody titer against Newcastle and influenza viruses as well as sheep red blood cell antigen were significantly (P<0.05) enhanced by feeding Harmal seed and extract. In conclusion, the results indicated that dietary inclusion of Harmal seed and extract enhanced immunological responses in broiler chicks.

Keywords: Broiler chicks, Harmal, immunity.

Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1099026

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[1] H.C. Wegener, F.M. Aarestrup, L.B. Jensen, A.M. Hammerum, F. Bager, “The association between the use of antimicrobial growth promoters and development of resistance in pathogenic bacteria towards growth promoting and therapeutic antimicrobials,” J. Anim. Feed Sci., vol. 7, pp.7-1, 1998.
[2] I. Phillips, M. Casewell, T. Cox, B. De Groot, C. Friis, R. Jones, C. Nightingale, R. Preston, J. Waddell, “Does the use of antibiotics in food animals pose a risk to human health? A critical review of published data” J. Antimicrobial Chemotherapy, vol. 53, pp. 28–52, 2004.
[3] M. K. Glynn, C. Bopp, W. Dewitt, P. Dabney, M. Mokhtar, F.J. Angulo, “Emergence of multidrug-resistant salmonella enterica serotype typhimurium DT104 infections in the United States,” New England J. Med., vol. 338, pp. 1333-1339, 1998.
[4] P. D. Fey, T. J. Safranek, M. E. Rupp, E.F. Dunne, E. Ribot, “Cefriaxone-resistant salmonella infection acquired by a child from cattle,” New England J. Med., vol. 432, pp. 1242-1249, 2000.
[5] H. J. D. Dorman, S. G. Deans, “Antimicrobial agents from plants: antimicrobial activity of plant volatile oils,” J. Appl. Microbiol., vol. 83, pp. 308-316, 2000.
[6] B.C. Arldogan, H. Baydar, S. Kaya, M. Demirci, D. Ozbasar, E.Mumcu, “Antimicrobial activity and chemical composition of some essential oils,” Arch. Pharmacol. Res., Vol 25, pp. 860-864, 2002.
[7] I.M.K. Abaza, M.A. Asar, G.E. Elshaarrawi, M.F. Hassan, “Effect of using nigella seeds,chamomile flowers, thyme flowers and harmal seeds as feed additives on performance of broiler,” Egypt. J. Agric. Res., vol. 81, pp. 735-750, 2003.
[8] V.F. Samanidou, E.N. Evaggelopoulou, “Chromatographic analysis of banned antibacterial growth promoters in animal feed,” J. Sep. Sci., vol. 31, pp. 2091-2112, 2008.
[9] M.M. Cowan, “Plant products as antimicrobial agents” Clinic. Microbiol. Rev. vol.12,pp. 564-582, 1999.
[10] M. Charai, M. Mosaddak, M. Faid, “Chemical composition and antimicrobial activities of two aromatic plants: Origanum majorana L. and O. compactum Benth,” J. Essential Oil Res., vol. 8, pp. 657–664, 1996.
[11] N. A. Botsoglou, P. Florou-Paneri, E. Christaki, D. J. Fletouris, A. B. Spais, “Effect of dietary oregano essential oil on performance of chickens and on iron-induced lipid oxidation of breast, thigh and abdominal fat tissues,” Brit. Poult. Sci., vol. 43, pp. 223-230, 2002.
[12] L. Korimova, J. Nagy, D. Mate, P. Korim, P. Turek, “Influence of rosemary and ascorbic acid on stability of fats in, Cingovska salama, stored in nonstandard conditions,” Slovenska Vet. J. vol. 28, pp. 35-36, 2003.
[13] K.H. Bang, D. W. Lee, H. M. Park, Y. H. Rhee, “Inhibition of fungal cell wall synthesizing enzymes by trans-cinnamaldehyde,” Biosci. Biotechnol. Biochem., vol 64,pp. 1061-1063, 2000.
[14] C. Bodinet, R. Mentel, U. Wegner, U. Lindequist, E. Teuscher, J. Freudenstine, “Effect of oral application of an immunomodulating plant extract on influenza type A infection in mice,” Planta Med., vol 68, pp. 896-900, 2002.
[15] K.R. Sharma, M. Agrawal, M.F. Marshall, “Heavy metals in vegetables collected from production and market sites of a tropical urban area of India,” Food Chem. Toxicol., vol. 47, pp. 583-591, 2009.
[16] P. Arumugam, N. Gayatri Priya, M. Subathra, A. Ramesh, “Antiinflammatory activity of four solvent fractions of ethanol extract of menthe spicata l. investigated on acute and chronic inflammation induced rats,” Environ. Toxicol. Pharmacol., Vol. 26, pp. 92-95, 2008.
[17] M. Toghyani, M. Toghyani, A. Gheisari, G. Ghalamkari, M. Mohammadrezaei, “Growth performance, serum biochemistry, and blood hematology of broiler chicks fed different levels of black seed (Nigella sativa) and peppermint (Mentha piperita),” Livest. Sci., vol. 129, pp. 173-178, 2010.
[18] N. Landy, G. Ghalamkari, M. Toghyani, “Performance, carcass characteristics, and immunity in broiler chickens fed dietary Neem (Azadirachta indica) as alternative for an antibiotic growth promoter,” Livest. Sci., vol. 142, pp. 305-309, 2011.
[19] T. Herraiz, D. Gonzalez, C. Ancin-Apilicueta, V. J. Aran, H. Guillen, “Beta-Carboline alkaloids in Pegannum harmala and inhibition of human monoamine oxidase (MAO),” Food Chem. Toxicol., vol. 48, pp. 839-45, 2010.
[20] L. Farouk, A. Laroubl, R. Aboufatima, A. Benharref, A. Chait, “Antinociceptive activity of various extracts of peganum harmala L. and possible mechanism of action,” Iran. J. Pharmacol. Therapeutics, vol. 8, pp. 29-35, 2008.
[21] G. Frison, D. Favretto, F. Zancanaro, G. Fazzin, S.D. Ferrara, “A case of bcarboline alkaloid intoxication following ingestion of Peganum harmala seed extract,” For. Sci. Int., vol. 179, pp. e37–e43, 2009.
[22] A.M. Soliman, S.R. Fahmy, “Protective and curative effects of the 15KD isolated protein from the Peganum harmala L.seeds against carbon tetrachloride oxidative stress in brain, tests and erythrocytes of rats,” Eur. Rev. Med. Pharmacol. Sci., vol. 15, pp. 888-90, 2011.
[23] A.F. Abdel-Fattah, K. Matsumoto, H.A. Gammaz, H. Watanabe, “Hypothermic effect of harmala alkaloid in rats: involvement of serotonergic mechanism,” Pharmacol. Biochem. Behav., vol. 52, pp. 421 – 6, 1995.
[24] H.R. Monsef, A. Ghobadi, M. Iranshahi, A. Abdollahi, “Antinociceptive effects of Peganum harmala L. alkaloid extract on mouse formalin test,” J. Pharmaceut. Sci., vol. 19, pp. 221-222, 2004.
[25] S.M. Adams, “The antineoplastic effects of prunusarmeniaca and Peganum harmala,” Dis. Abstr. Int. Sci., vol. 44, pp. 1052- 1055, 1983.
[26] A.R. Shahverdi, H. R. Monsef-Esfahani, B. Nickavar, L. Bitarafan, S. Khodaee, N. Khoshakhlagh, “Antimicrobial activity and main chemical composition of two smoke condensates from Peganum harmala seeds. Z. Naturforsch,” Chinese J. Biosci., vol. 60, pp.707–710, 2005.
[27] K. Hamdan, H. Masmoudi, F. Ellouz, A. ElFeki, S. Carreau, “Protective effects of Peganum harmala extracts on thiourea-induced diseases in adult male rat” J. Env. Bio., vol. 29, pp. 73-77, 2008.
[28] S.Q. Walid, “The effect of low levels of dietary Peganum harmala L. and Ballota undulata or their mixture on chicks,” J. Anim. Vet. Adv., vol. 8, 1535-1538, 2009.
[29] SAS Institute, “SAS User’s Guide,” Version 8.02 ed. SAS Institute Inc., Cary, NC. 2001.
[30] H. Berrougui, M. Isabelle, M. Cloutier, M. Hmamouchi, A. Khalil, “Protective effects of Peganum harmala L. extract, harmine and harmaline against human low–desity lipoprotein oxidation,” J. Pharmacy & Pharamacol., vol. 58, pp. 967-974, 2006.
[31] J.C. Callaway, D.J. McKenna, C.S. Grob, G.S. Brito, L.P. Raymon, R.E.Poland, E.N. Andrade, E.O. Andrade, D.C. Mash, “Pharmacokinetics of hoasca alkaloids in healthy humans,” J. Ethnopharmacol. vol. 65, pp. 243–256, 1999.
[32] D.J. McKenna, J.C. Callaway, C.S. Grob, “The sciencific investigation of Ayahuasca: a review of past and current research,” Heffter Rev. Psychedelic Res. Vol. 1, pp. 65–76, 1998.
[33] M.B.H. Youdim, D. Edmondson, K.F. Tipton, “The therapeutic potential of monoamine oxidase inhibitors,” Nat. Rev. Neurosci. Vol. 7, pp. 295– 309, 2006.
[34] T. Herraiz, C. Chaparro, “Human monoamine oxidase is inhibited by tobacco smoke: b-carboline alkaloids act as potent and reversible inhibitors,” Biochem. Biophys. Res. Commun. Vol. 326, pp. 378–386, 2005.
[35] T. Herraiz, C. Chaparro, “Analysis of monoamine oxidase enzymatic activity by reversed-phase high performance liquid chromatography and inhibition by b-carboline alkaloids occurring in foods and plants,” J. Chromatogr. Vol. 1120, pp. 237– 243, 2006a.
[36] T. Herraiz, C. Chaparro, “Human monoamine oxidase enzyme inhibition by coffee and b-carbolines norharman and harman isolated from coffee,” Life Sci. vol.78, pp. 795–802, 2006b.