Aftab Ahmad Anjum

Abstracts

2 Experimental Lead Toxicity in Lohi Sheep: Risks and Impact on Edible Tissues

Authors: Raheela Akhtar, Muhammad Younus, Aman Ullah Khan, Aftab Ahmad Anjum, Muti-Ur-Rehman Khan, Muhammad Sajid, Muhammad Asif Idrees, Iahtasham Khan, Sajid Umar

Abstract:

The present study was conducted to investigate the hazardous effects of lead on health and edible organs of Lohi sheep. The adult Lohi sheep (n=48) were randomly divided into two equal groups. The first group was administered lead acetate at dose of 70 mg/kg live body weight daily as 10% solution by oral route for a period of 90 days and the second group served as a negative control. Blood and tissue samples were collected at day 0, 30, 60 and 90 and analyzed for lead concentration by atomic absorption spectrophotometry. The kidney showed the highest lead concentration (p < 0.05) followed by liver and then muscle. Lead acetate treated sheep showed structural and behavioral changes during the last month of trial. Liver showed necrosis, hemorrhages and hyperactivation of macrophages. Kidney showed degenerative and necrotic changes in glomeruli and tubules and the characteristic intranuclear inclusion bodies in tubular epithelial cells on H and E staining. It was concluded that Lohi sheep is affected by lead intoxication at low dose for longer period and hence exhibits lead accumulation in edible tissues.

Keywords: Histopathology, Lohi sheep, lead acetate, edible tissue

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1 Differential Antibrucella Activity of Bovine and Murine Macrophages

Authors: Raheela Akhtar, Muhammad Younus, Zafar Iqbal Chaudhary, Yongqun Oliver He, Aftab Ahmad Anjum

Abstract:

Brucella abortus is an intracellular pathogen affecting macrophages. Macrophages release some components such as lysozymes (LZ), reactive oxygen species (ROS) and reactive nitrite intermediates (RNI) which are important tools against intracellular survival of Brucella. The antibrucella activity of bovine and murine macrophages was compared following stimulation with Brucella abortus lipopolysaccharides. Our results revealed that murine macrophages were ten times more potent to produce antibrucella components than bovine macrophages. The differential production of these components explained the differential Brucella killing ability of these species that was measured in terms of intramacrophagic survival of Brucella in murine and bovine macrophages.

Keywords: Cytokines, bovine macrophages, Brucella abortus, cell stimulation, Murine macrophages

Procedia PDF Downloads 349