Rehab F. Abdel-Rahman

Abstracts

3 In vivo Evaluation of the Anti-Inflammatory, Analgesic Activities of Crataegus Sinaica Methanol Extract

Authors: Rehab F. Abdel-Rahman, A. H. El-Desoky, Gihan F. Asaad

Abstract:

Objective: The research was designed to study the anti-inflammatory and analgesic effect of Crataegus sinaica methanol extract Methods: The anti-inflammatory were measured by edema rate and inhibition rate using plethysmometer and by determinig PGE2, TNF-ɑ and MPO levels in paw exudate. Both central and peripheral analgesic effect were examined using Writhing test and hot plate test Key Findings: The methanolic extract showed a significant anti-inflammatory effect by decreasing paw edema induced by carrageenan; also results showed that anti-inflammatory effect increased by increasing the dose. All dose levels of the extract showed significant reduction in Prostaglandin E2, Tumor necrosis factor-ɑ, and Meyloperoxidase levels. Crataegus sinaica methanol extract showed both peripheral and central analgesic effect. Conclusion: The C. sinaica methanolic extract can be considered a promising anti-inflammatory and analgesic product.

Keywords: Pain, Analgesia, prostaglandins, edema, Crataegus sinaica, hot plate

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2 Neuroprotective Effect of Vildagliptin against Cerebral Ischemia in Rats

Authors: Rehab F. Abdel-Rahman, Salma A. El-Marasy, Reham M. Abd-Elsalam

Abstract:

The burden of stroke is intensely increasing worldwide. Brain injury following transient or permanent focal cerebral ischemia develops ischemic stroke as a consequence of a complex series of pathophysiological events. The aim of this study is to evaluate the possible neuroprotective effect of a dipeptidyl peptidase-4 inhibitor, vildagliptin, independent on its insulinotropic properties in non-diabetic rats subjected to cerebral ischemia. Anaesthetized Wistar rats were subjected to either left middle cerebral artery occlusion (MCAO) or sham operation followed by reperfusion after 30 min of MCAO. The other three groups were orally administered vildagliptin at 3 dose levels (2.5, 5, 10 mg/kg) for 3 successive weeks before subjected to left focal cerebral ischemia/reperfusion and till the end of the study. Neurological deficit scores and motor activity were assessed 24h following reperfusion. 48h following reperfusion, rats were euthanized and their left brain hemispheres were harvested and used in the biochemical, histopathological, and immunohistochemical investigations. Vildagliptin pretreatment improved neurological score deficit, locomotor activity and motor coordination in MCAO rats. Moreover, vildagliptin reduced malondialdehyde (MDA), elevated reduced glutathione (GSH), phosphotylinosital 3 kinase (PI3K), phosphorylated of protein kinase B (p-AKT), and mechanistic target of rapamycin (mTOR) brain contents in addition to reducing protein expression of caspase-3. Also, vildagliptin showed a dose-dependent attenuation in neuronal cell loss and histopathological alterations in MCAO rats. This study proves that vildagliptin exerted the neuroprotective effect in a dose-dependent manner as shown in amelioration of neuronal cell loss and histopathological damage in MCAO rats, which may be mediated by attenuating neuronal and motor deficits, it’s anti-oxidant property, activation of PI3K/AKT/mTOR pathway and its anti-apoptotic effect.

Keywords: rats, Oxidative Stress, caspase-3, cerebral ischemia, dipeptidyl peptidase-4 inhibitor, PI3K/AKT/mTOR pathway, vildagliptin

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1 Neuroprotective Effect of Crocus sativus against Cerebral Ischemia in Rats

Authors: Sally A. El Awdan, Dina F. Mansour, Rehab F. Abdel-Rahman, Rehab R. Hegazy, Hanan A. Ogaly, Marwan Abdelbaset

Abstract:

Disorders of the cerebral circulation are the leading cause of numerous neurological and psychiatric illnesses. The transient middle cerebral artery occlusion model (MCAO) is considered to be a reliable and reproducible rodent model of cerebral ischemia. The purpose of the current study was to examine the neuroprotective effects of Crocus sativus (saffron) in a rat model of left middle cerebral artery MCAO. Male Wistar rats were anesthetized and subjected to 1 h of MCAO followed by 48 h reperfusion or sham surgery. One group of the ischemia operated animals was kept as left brain ischemia/reperfusion (I/R). Another 2 operated groups received saffron extract (100 or 200 mg/kg, i.p) four times (60 min before the surgery, during the surgery, and on days 1 and 2 after the occlusion). During the experiment, behavioral tests were performed. After 72 h the animals were euthanized and their left brain hemispheres were used in the biochemical, histopathological, and immunohistochemical studies. Saffron administration revealed an improvement in I/R-induced alteration of locomotor balance and coordination ability of rats. Moreover, saffron decreased the brain content of malondialdehyde, nitric oxide, brain natriuretic peptide and vascular endothelial growth factor with significant increase of reduced glutathione. Immunohistochemical evaluation of caspase-3 and Bax protein expression revealed reduction in I/R-enhanced apoptosis in saffron treated rats. In conclusion, saffron treatment decreases ischemic brain injury in association with inhibition of apoptotic and oxidative cell death in a dose dependent manner.

Keywords: rats, caspase-3, vascular endothelial growth factor, cerebral ischemia, Crocus sativus

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