TY - JFULL AU - Mustafa Erat PY - 2014/11/ TI - The Influence of Some Polyphenols on Human Erythrocytes Glutathione S-Transferase Activity T2 - International Journal of Bioengineering and Life Sciences SP - 697 EP - 702 VL - 8 SN - 1307-6892 UR - https://publications.waset.org/pdf/9999396 PU - World Academy of Science, Engineering and Technology NX - Open Science Index 94, 2014 N2 - Glutathione S-transferase was purified from human erythrocytes and effects of some polyphenols were investigated on the enzyme activity. The purification procedure was performed on Glutathione-Agarose affinity chromatography after preparation of erythrocytes hemolysate with a yield of 81%. The purified enzyme showed a single band on the SDS-PAGE. The effects of some poliphenolic compounds such as catechin, dopa, dopamine, progallol and catechol were examined on the in vitro GST activity. Catechin was determined to be inhibitor for the enzyme, but others were not effective on the enzyme as inhibitors or activators. IC50 value -the concentration of inhibitor which reduces enzyme activity by 50%- was estimated to be 10 mM. Ki constants were also calculated as 6.38 ± 0,70 mM with GSH substrate, and 3.86 ± 0,78 mM with CDNB substrate using the equations of graphs for the inhibitor, and its inhibition type was determined as non-competitive. ER -