TY - JFULL AU - Masoumeh Ghasemi and Afshin Farahbakhsh and Arman Farahbakhsh and Ali Asghar Safari PY - 2014/10/ TI - Using Submerge Fermentation Method to Production of Extracellular Lipase by Aspergillus niger T2 - International Journal of Bioengineering and Life Sciences SP - 1019 EP - 1023 VL - 8 SN - 1307-6892 UR - https://publications.waset.org/pdf/9999378 PU - World Academy of Science, Engineering and Technology NX - Open Science Index 93, 2014 N2 - In this study, lipase production has been investigated using submerge fermentation by Aspergillus niger in Kilka fish oil as main substrate. The Taguchi method with an L9 orthogonal array design was used to investigate the effect of parameters and their levels on lipase productivity. The optimum conditions for Kilka fish oil concentration, incubation temperature and pH were obtained 3 gr./ml 35°C and 7, respectively. The amount of lipase activity in optimum condition was obtained 4.59IU/ml. By comparing this amount with the amount of productivity in the olive oil medium based on the cost of each medium, it was that using Kilka fish oil is 84% economical. Therefore Kilka fish oil can be used as an economical and suitable substrate in the lipase production and industrial usages. ER -