@article{(Open Science Index):https://publications.waset.org/pdf/9999276,
	  title     = {Stroma-Providing Activity of Adipose Derived Mesenchymal Stromal Cells in Tissue-Related O2 Microenvironment},
	  author    = {P. I. Bobyleva and  E. R. Andreeva and  I. V. Andrianova and  E. V. Maslova and  L.B. Buravkova},
	  country	= {},
	  institution	= {},
	  abstract     = {This work studied the ability of adipose tissue-derived
mesenchymal stromal cells (MSCs) to form stroma for expansion of
cord blood hematopoietic cells. We showed that 72-hour interaction
of MSCs with cord blood mononuclear cells (MNCs) in vitro at
atmospheric (20%) and low (5%) O2 conditions increased the
expression of ICAM-1, HCAM (at the beginning of interaction) on
MSCs. Viability of MSCs and MNCs were maintained at high level.
Adhesion of MNCs to MSCs was faster at 20% O2. MSCs promoted
the proliferation of adhered MNCs to form the suspension containing
great number of hematopoietic colony-forming units, and this effect
was more pronounced at 5% O2. Thus, adipose-derived MSCs
supplied sufficient stromal support to cord blood MNCs both at 20%
and 5% О2, providing their adhesion with further expansion of new
generation of different hematopoietic lineages.
},
	    journal   = {International Journal of Bioengineering and Life Sciences},
	  volume    = {8},
	  number    = {9},
	  year      = {2014},
	  pages     = {544 - 548},
	  ee        = {https://publications.waset.org/pdf/9999276},
	  url   	= {https://publications.waset.org/vol/93},
	  bibsource = {https://publications.waset.org/},
	  issn  	= {eISSN: 1307-6892},
	  publisher = {World Academy of Science, Engineering and Technology},
	  index 	= {Open Science Index 93, 2014},
	}