Sudip Ghosh and Dipanwita Roy and Dipan Chatterjee and Paramita Bhattacharjee and Satadal Das
SFE as a Superior Technique for Extraction of EugenolRich Fraction from Cinnamomum tamala Nees (Bay Leaf) Process Analysis and Phytochemical Characterization
44 - 52
2014
8
1
International Journal of Nutrition and Food Engineering
https://publications.waset.org/pdf/9997279
https://publications.waset.org/vol/85
World Academy of Science, Engineering and Technology
Highest yield of eugenolrich fractions from Cinnamomum tamala (bay leaf) leaves were obtained by supercritical carbon dioxide (SCCO2), compared to hydrodistillation, organic solvents, liquid CO2 and subcritical CO2 extractions. Optimization of SCCO2 extraction parameters was carried out to obtain an extract with maximum eugenol content. This was achieved using a sample size of 10g at 55°C, 512 bar after 60min at a flow rate of 25.0 cm3sof gaseous CO2. This extract has the best combination of phytochemical properties such as phenolic content (1.77mg gallic acidg dry bay leaf), reducing power (0.80mg BHTg dry bay leaf), antioxidant activity (IC50 of 0.20mgml) and antiinflammatory potency (IC50 of 1.89mgml). Identification of compounds in this extract was performed by GCMS analysis and its antimicrobial potency was also evaluated. The MIC values against E. coli, P. aeruginosa and S. aureus were 0.5, 0.25 and 0.5mgml, respectively.
Open Science Index 85, 2014