{"title":"SFE as a Superior Technique for Extraction of Eugenol-Rich Fraction from Cinnamomum tamala Nees (Bay Leaf) - Process Analysis and Phytochemical Characterization","authors":"Sudip Ghosh, Dipanwita Roy, Dipan Chatterjee, Paramita Bhattacharjee, Satadal Das","volume":85,"journal":"International Journal of Nutrition and Food Engineering","pagesStart":44,"pagesEnd":53,"ISSN":"1307-6892","URL":"https:\/\/publications.waset.org\/pdf\/9997279","abstract":"
Highest yield of eugenol-rich fractions from Cinnamomum tamala<\/i> (bay leaf) leaves were obtained by supercritical carbon dioxide (SC-CO2<\/sub>), compared to hydro-distillation, organic solvents, liquid CO2<\/sub> and subcritical CO2 <\/sub>extractions. Optimization of SC-CO2<\/sub> extraction parameters was carried out to obtain an extract with maximum eugenol content. This was achieved using a sample size of 10g at 55<\/span>°<\/span>C, 512 bar after 60min at a flow rate of 25.0 cm3<\/sup>\/sof gaseous CO2<\/sub>. This extract has the best combination of phytochemical properties such as phenolic content (1.77mg gallic acid\/g dry bay leaf), reducing power (0.80mg BHT\/g dry bay leaf), antioxidant activity (IC50<\/sub> of 0.20mg\/ml) and anti-inflammatory potency (IC50<\/sub> of 1.89mg\/ml). Identification of compounds in this extract was performed by GC-MS analysis and its antimicrobial potency was also evaluated. The MIC values against E. coli<\/i>, P. aeruginosa<\/i> and S. aureus<\/i> were 0.5, 0.25 and 0.5mg\/ml, respectively<\/span>.