%0 Journal Article
	%A Sudip Ghosh and  Dipanwita Roy and  Dipan Chatterjee and  Paramita Bhattacharjee and  Satadal Das
	%D 2014
	%J International Journal of Nutrition and Food Engineering
	%B World Academy of Science, Engineering and Technology
	%I Open Science Index 85, 2014
	%T SFE as a Superior Technique for Extraction of Eugenol-Rich Fraction from Cinnamomum tamala Nees (Bay Leaf) - Process Analysis and Phytochemical Characterization
	%U https://publications.waset.org/pdf/9997279
	%V 85
	%X Highest yield of eugenol-rich fractions from Cinnamomum tamala (bay leaf) leaves were obtained by supercritical carbon dioxide (SC-CO2), compared to hydro-distillation, organic solvents, liquid CO2 and subcritical CO2 extractions. Optimization of SC-CO2 extraction parameters was carried out to obtain an extract with maximum eugenol content. This was achieved using a sample size of 10g at 55°C, 512 bar after 60min at a flow rate of 25.0 cm3/sof gaseous CO2. This extract has the best combination of phytochemical properties such as phenolic content (1.77mg gallic acid/g dry bay leaf), reducing power (0.80mg BHT/g dry bay leaf), antioxidant activity (IC50 of 0.20mg/ml) and anti-inflammatory potency (IC50 of 1.89mg/ml). Identification of compounds in this extract was performed by GC-MS analysis and its antimicrobial potency was also evaluated. The MIC values against E. coli, P. aeruginosa and S. aureus were 0.5, 0.25 and 0.5mg/ml, respectively. 

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