TY - JFULL AU - E. Mansyah and Sobir and E. Santosa and A. Sisharmini and Sulassih PY - 2012/12/ TI - Polymorphic Marker Designed from Bioinformatics Sequences Related to Cell Wall Strength for Discrimination of Mangosteen (Garcinia mangostana L.) Clones Resistant to Gamboge Disorder T2 - International Journal of Agricultural and Biosystems Engineering SP - 1022 EP - 1028 VL - 6 SN - 1307-6892 UR - https://publications.waset.org/pdf/8291 PU - World Academy of Science, Engineering and Technology NX - Open Science Index 71, 2012 N2 - Gamboge disorder (GD) or fruit damage by the yellow sap is a major problem in mangosteen. Mangosteen plants varied in the level of GD, from very low or non GD to low, moderate and high GD. However it was difficult to differentiate between GD and non GD plants because evaluation of the disorder is strongly influenced by environment. In this study we investigated the usefulness of primer designed from bioinformatics related to cell wall strength, termed as MCWS, to predict GD. Plant materials used were 28 mangosteen plants selected based on percentage of GD categorized as high, moderate, low and very low or non GD. The result showed that the specific DNA fragments were absent in the high GD accessions. The MCWS marker suggests as a novel polymorphic marker for GD in mangosteen as well as a marker for detect variability in mangosteen as apomictic plant. ER -