Effect of Recombinant Human Follicle Stimulating Hormone on Meiotic Competence of In Vitro Grown Nili Ravi Buffalo Oocytes

Authors: Muhammad Ijaz Khan, Samina Jalali, Beenish Shahid, S. A. Shami, Muhammad Ikramullah

Abstract:

In the present study, the response of Nili Ravi buffalo oocytes to recombinant human follicle stimulating hormone (rhFSH) (Organon) on meiotic maturation in vitro was examined. Oocytes were matured in vitro in medium containing either 0 or 0.05 IU/ ml rhFSH and the stage of nuclear maturation recorded after 24 hours. The percentage of oocytes in the control group undergoing germinal vesicle breakdown (GVBD) observed after 24 hours of culture was 29 % whereas as in rhFSH group the percentage was 10 % were at this stage (P< 0.001).Thus in the presence of rhFSH, a significantly greater number of oocytes had progressed to the more advanced stages of nuclear maturation. Indeed, the maturation of GV (Germinal Vesicle) stage oocytes to the metaphase II (M II) stage after 24 hours was significantly (P< 0.0001) increased by the addition of rhFSH (82 % VS 47 %). The percentage of degenerated oocytes after 24 hours of culture was 24 % in control group, whereas in rhFSH group the percentage was 8 % after 24 hours. Degeneration of the oocytes after 24 hours was not significantly (P = 0. 9361) decreased.

Keywords: Buffalo, in vitro, oocytes, recombinant FSH.

Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1326712

References:

[1] D.C. Michle, A.N., Caveney, L.C., Smith, A.J. and Watson, "Responsiveness of bovine cumulus-oocytes complexes to porcine and recombinant human FSH, and the effect of COc quality on gonadotropins," 1: (14)-2003.
[2] A.B. Galway, P.S. Lapolt, A. Tsafriri, et al., "Recombinant follicle stimulating hormone induces ovulation and tissue plasminogen activator expression in hypophysectomized rats," Endocrionology; 127, (3023- 3028) - 1990.
[3] J. Tornell, C. Bergh and U. Selleskog, 1995. Effect of recombinant human Gonadotrophins on oocyte meiosis and steroidogenesis in isolated pre-ovulatory rat follicles," Hum. Reprod. 10, (1619-1622)- 1995.
[4] A.G. Byskov, C. Yding Anderson, A. Hossaini, et al, "Cumulus cells of oocyte cumulus complexes secrete a meiosis-activating substance when stimulated with FSH," Mol. Repod. Dev; 46, (296-305)-1997.
[5] D Rizos, F. Ward, P. Duffy, M.P. Boland, and P. Lonergan, "Consequences of bovine oocyte maturation, fertilization or early embryo development in vitro versus in vivo: Implications for blastocyst yield and blastocyst yield and blastobyst quality," Mol. Reprod. Dev. 61, (234-248)-2002.
[6] P. Sutovsky, J.E. Flechon, and A. Pavlok, "Microfilaments, Microtubules and intermediate filaments fulfill different roles in gonadotropin induced expansion of bovine cumulus oophorus," Reprod. Nutr. Dev. 34, (415-425) 1994.
[7] G. Pincus, and E.V. Enzmann, "The comparative behaviour of mammalian egg in-vivo and in-vitro," I. The activation of ovarian eggs. J. Exp. Med. 62, (665-675)-1935.
[8] A.C. Schroeder, and J.J. Eppig, "The development capacity of mouse oocytes that matured spontaneously in-vitro is normal," Dev. Biol. 102, 493-497 - 1984.
[9] K. Saeki, M. Hoshi, M.L. Rutledge, "In-vitro fertilization and development of bovine oocytes matured with commercially available follicle stimulating hormones," Theriogenology. 34, (1035-1039)-1990.
[10] M. Fukushima, and Y Fukui, "Effect of gonadotrophins and steroids on the subsequent fertilizability of extra follicular bovine oocyte culture in vitro," Anim. Reprod. Sci. 9, (323-332)-1985.
[11] H.H.Christ, K. Joachim, L. Monika, G. Christina, O. Jan, M.B. Henning, E. Sabine and E.R. Ursula, . "Nuclear and cytoplasmic maturation of mouse oocytes after treatment with synthetic meiosisactivating sterol in vitro," Biol. Reprod. 61, (1362-1372)-1999.
[12] J.J. Eppig, J.J., O, Brien , and K. Wigglesworth , "Mammalian oocyte growth and development in vitro," Mol. Repord . Dev. 44, (260-273)- 1996.
[13] S.K. Roy, and B.J. Treacy, "Isolation and Long-term culture of human preantral follicles," Fertil. Steril. 59 (suppl4), (783 -790)-1993.
[14] C. Sandra, B. Barbara, C. Mimi, And M.M Maruo, "In vitro development of sheep preantral follicles," Biol. Reprod. 60, (594)-1999.
[15] A.I. Younis, B.G Brackett, and R.A. Hosken, "Influence of serum and hormones on bovine oocytes maturation and fertilization in -vitro," Gamete Res. 23, (189-201)-1989.
[16] K.A. Zuelke, A.I. Younis, .and B.G. Brackett, "Enhanced oocytes maturation of bovine oocytes with and with protein supplementation. Serono Symposium on Fertilization in Mammals," Boston, MA. p.47, abs 1989.
[17] C. Anderiesz, A. Ferraretti, C. Magli, A. Fiorentino, D. Fortini, L. Gianaroli, G.M. Jones, and A.O.Trounson, "Effect of recombinant human Gonadotrophins on human, bovine and murine oocyte meiosis, fertilization and embryonic development," Hum. Reprod. (15:1148)- 2000.
[18] J.L. Tremoleda, T. Tharasanit, H.A.T.Van Tol, S.T.E. Stout, B. Colenbrander, and M.N. Bevers, "Effects of follicular cells and FSH on the resumption of meiosis in equine oocytes matured in vitro," Biol. Reprod. 125, (665-577)-2003.
[19] D.T. Armstrong, X. Zhang, and B.C. Vanderhyder, "Hormonal actions during oocyte maturation influence, fertilization and early embryonic development," Ann. N. Y Acad.Sci. 626, (137-158)-1991.
[20] R.G. Cortvrind, Y. Hu, Gunliu, and E.J. Gohan, "Timed analysis of the nuclear maturation of oocytes in early preantral mouse follicle culture supplemented with recombinant gonadotrophins," Fertil. Steril. 70, (1114-1125) -1998.