Shou-Chen Lo and Chia-Ching Lin and Chieh-Chen Huang
Enhance Halorespiration in Rhodopseudomonas palustris with Cytochrome P450cam System from Pseudomonas putida
307 - 313
2012
6
5
International Journal of Bioengineering and Life Sciences
https://publications.waset.org/pdf/7668
https://publications.waset.org/vol/65
World Academy of Science, Engineering and Technology
To decompose organochlorides by bioremediation, coculture biohydrogen producer and dehalogenation microorganisms is a useful method. In this study, we combined these two characteristics from a biohydrogen producer, Rhodopseudomonas palustris, and a dehalogenation microorganism, Pseudomonas putida, to enchance halorespiration in R. palustris. The genes encoding cytochrome P450cam system (camC, camA, and camB) from P. putida were expressed in R. palustris with designated expression plasmid. All tested strains were cultured to log phase then presented pentachloroethane (PCA) in media. The vector control strain could degrade PCA about 78 after 16 hours, however, the cytochrome P450cam system expressed strain, CGAcamCAB, could completely degrade PCA in 12 hours. While taking chlorinated aromatic, 3chlorobenzoate, as sole carbon source or present benzoate as cosubstrate, CGAcamCAB presented faster growth rate than vector control strain.
Open Science Index 65, 2012