T. Bovornvirakit and K. Viravaidya
Development of an Immunoassay Platform for Diagnosis of Acute Kidney Injury
485 - 489
2011
5
10
International Journal of Biomedical and Biological Engineering
https://publications.waset.org/pdf/7578
https://publications.waset.org/vol/58
World Academy of Science, Engineering and Technology
Acute kidney injury (AKI) is a new worldwide public
health problem. A diagnosis of this disease using creatinine is still a
problem in clinical practice. Therefore, a measurement of biomarkers
responsible for AKI has received much attention in the past couple
years. Cytokine interleukin18 (IL18) was reported as one of the
early biomarkers for AKI. The most commonly used method to
detect this biomarker is an immunoassay. This study used a planar
platform to perform an immunoassay using fluorescence for
detection. In this study, antiIL18 antibody was immobilized onto a
microscope slide using a covalent binding method. Makeup samples
were diluted at the concentration between 10 to 1000 pgml to create
a calibration curve. The precision of the system was determined
using a coefficient of variability (CV), which was found to be less
than 10. The performance of this immunoassay system was
compared with the measurement from ELISA.
Open Science Index 58, 2011