TY - JFULL AU - T. Bovornvirakit and K. Viravaidya PY - 2011/11/ TI - Development of an Immunoassay Platform for Diagnosis of Acute Kidney Injury T2 - International Journal of Biomedical and Biological Engineering SP - 484 EP - 489 VL - 5 SN - 1307-6892 UR - https://publications.waset.org/pdf/7578 PU - World Academy of Science, Engineering and Technology NX - Open Science Index 58, 2011 N2 - Acute kidney injury (AKI) is a new worldwide public health problem. A diagnosis of this disease using creatinine is still a problem in clinical practice. Therefore, a measurement of biomarkers responsible for AKI has received much attention in the past couple years. Cytokine interleukin-18 (IL-18) was reported as one of the early biomarkers for AKI. The most commonly used method to detect this biomarker is an immunoassay. This study used a planar platform to perform an immunoassay using fluorescence for detection. In this study, anti-IL-18 antibody was immobilized onto a microscope slide using a covalent binding method. Make-up samples were diluted at the concentration between 10 to 1000 pg/ml to create a calibration curve. The precision of the system was determined using a coefficient of variability (CV), which was found to be less than 10%. The performance of this immunoassay system was compared with the measurement from ELISA. ER -