@article{(Open Science Index):https://publications.waset.org/pdf/7578, title = {Development of an Immunoassay Platform for Diagnosis of Acute Kidney Injury}, author = {T. Bovornvirakit and K. Viravaidya}, country = {}, institution = {}, abstract = {Acute kidney injury (AKI) is a new worldwide public health problem. A diagnosis of this disease using creatinine is still a problem in clinical practice. Therefore, a measurement of biomarkers responsible for AKI has received much attention in the past couple years. Cytokine interleukin-18 (IL-18) was reported as one of the early biomarkers for AKI. The most commonly used method to detect this biomarker is an immunoassay. This study used a planar platform to perform an immunoassay using fluorescence for detection. In this study, anti-IL-18 antibody was immobilized onto a microscope slide using a covalent binding method. Make-up samples were diluted at the concentration between 10 to 1000 pg/ml to create a calibration curve. The precision of the system was determined using a coefficient of variability (CV), which was found to be less than 10%. The performance of this immunoassay system was compared with the measurement from ELISA.}, journal = {International Journal of Biomedical and Biological Engineering}, volume = {5}, number = {10}, year = {2011}, pages = {485 - 489}, ee = {https://publications.waset.org/pdf/7578}, url = {https://publications.waset.org/vol/58}, bibsource = {https://publications.waset.org/}, issn = {eISSN: 1307-6892}, publisher = {World Academy of Science, Engineering and Technology}, index = {Open Science Index 58, 2011}, }