The Study of the Interaction between Catanionic Surface Micelle SDS-CTAB and Insulin at Air/Water Interface
Herein, we report the different types of surface morphology due to the interaction between the pure protein Insulin (INS) and catanionic surfactant mixture of Sodium Dodecyl Sulfate (SDS) and Cetyl Trimethyl Ammonium Bromide (CTAB) at air/water interface obtained by the Langmuir-Blodgett (LB) technique. We characterized the aggregations by Scanning Electron Microscopy (SEM), Atomic Force Microscopy (AFM) and Fourier transform infrared spectroscopy (FTIR) in LB films. We found that the INS adsorption increased in presence of catanionic surfactant at air/water interface. The presence of small amount of surfactant induces two-stage growth kinetics due to the pure protein absorption and protein-catanionic surface micelle interaction. The protein remains in native state in presence of small amount of surfactant mixture. Smaller amount of surfactant mixture with INS is producing surface micelle type structure. This may be considered for drug delivery system. On the other hand, INS becomes unfolded and fibrillated in presence of higher amount of surfactant mixture. In both the cases, the protein was successfully immobilized on a glass substrate by the LB technique. These results may find applications in the fundamental science of the physical chemistry of surfactant systems, as well as in the preparation of drug-delivery system.
Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1333885Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 2078
 P. Zimmet, K. G.Alberti, J.Shaw, "Global and societal implications of the diabetes epidemic", Nature, vol. 414, 782-787, Dec, 2001.
 S.Wild, G.Roglic, A.Green, R.Sicree, H.King, "Global Prevalence of Diabetes Estimates for the year 2000 and projections for 2030", Diabetes Care, vol. 27, pp. 1047-1053, 2004.
 International Diabetes Federation. http://www.idf.org. Accessed Nov 22, 2010.
 WHO Diabetes. http://www.who.int/diabetes. Accessed Nov 22, 2010.
 J.Kraineva, V.Smirnovas, R.Winter, "Effects of Lipid Confinement on Insulin Stability and Amyloid Formation" Langmuir, vol. 23, pp. 7118- 7126, 2007.
 C. Chen-Shang.Wu, J.T. Yang, "Conformation of insulin and its fragments in surfactant solutions" Biochimica et Biophysica Acta, vol. 667, pp. 285-293, 1981.
 S. P. Lopez, N.M.B.Vila, N.V. Romeu, "Bovine Insulinosphatidylcholine Mixed Langmuir Monolayers: Behavior at the Air- Water Interface", Journal of Physical. Chemistry B, vol. 115 pp. 9387- 9394, 2011.
 J.M. Thornton, "Disulphide Bridges in Globular Proteins", Journal of Molecular Biology, vol. 151, pp. 261-287, 1981.
 J. Brange, U. Ribel, J. F. Hansen, G. Dodson, M. T. Hansen, S. Havelund, S. G. Melberg, F. Norris, K. Norris, L. Snel, A. R. S├©rensen, H. O. Voigt, "Monomeric insulins obtained by protein engineering and their medical implications", Nature, vol. 333, pp. 679-682, 1988.
 J. Gunning, T. Blundell, The Peptides. Analysis, Synthesis, Biology;Academic: New York, vol. 4, pp. 55-84, 1981.
 T. L. Blundeil, G. G. Dodson, D. C. Hodgkin, D. A. Mercola, Adv. Protein Chem , "Insulin: The Structure in the Crystal and its Reflection in Chemistry and Biology" vol. 26, pp. 279-402, 1972.
 Peking Insulin Stzueture Research Group, "Studies on the insulin crystal structure: the molecule at 1.8A resolution", Scientia Sinlea, vol. 17, pp. 752-778.
 J. L. Whittingham, D. J. Scott, K. Chance, A. Wilson, J. Finch, J. Brange, G. G. Dodson, "Insulin at pH 2: Structural Analysis of the conditions Promoting Insulin Fibre Formation" Journal of Molecular Biology, vol. 318, pp. 479-490, 2002.
 Y. W. Chien, "Human insulin: Basic Sciences and Therapeutic uses", Drug Development and Industrial Pharmacy, vol. 22, pp. 753-789, 1996.
 G. M. Grodsky, P. H. Forsham, "Insulin and the Pancreas", Annual Review of Physiology, vol. 28, pp. 347-380, 1966.
 D.F. Steiner, "The Biosynthesis of Insulin- Genetic, Evolutionary, and Pathophysiologic Aspects", Harvey Lectures, vol. 78, pp. 191-228, 1983.
 D.F. Steiner, "The banting memorial lecture 1976. Insulin today", Diabetes, vol. 26, pp. 322-331, 1997.
 R. Hanas, J. Ludvigsson, "Experience of pain from insulin injections and needlephobia in young patients with IDDM", Practical Diabetes International, vol. 14, pp. 95-99, 2005.
 Z. Shao, Y. Li, T. Chermak,; A. K. Mitra, "Cyclodextrins as mucosal absorption promoters of insulin: II. Effects of betacyclodextrin derivatives on alpha-chymotryptic degradation and enteral absorption of insulin in rats", Pharmaceutical Research, vol. 11, pp. 1174-1179,1994.
 N. Jerry, Y.Anitha, C. P. Sharma, P. Sony, "In vivo absorption studies of insulin from an oral delivery system", Drug Delivery, vol. 8, pp. 19, 2001.
 Y. Z. Li, Y. S. Quan, L. Zang, M. N. Jin, F. Kamiyama, H. Katsumi, A.Yamamoto, S. Tsutsumi, Biol. Pharm. Bull, "Transdermal Delivary of insulin using trypsin as a biochemical enhancer", vol. 31 pp. 1574-1579, 2008.
 D. R. Owens, B. Zinman, G. Bolli, "Alternative routes of insulin delivery", Diabetic Medicine, vol. 20, pp. 886-898, 2003.
 G. P. Carino, E. Mathiowitz, "Oral insulin delivery", Advanced Drug Delivery Reviews, vol. 35, pp. 249-257, 1999.
 M. Berger, F. Eds. Gries, Oral Insulin 1922-1992: the History of Continuous Ambition and Failure. In Frontiers in Insulin Pharmacology, Plenum Press: Stuttgart, Germany, vol. 144, 1993.
 M. N. Su├írez, N. V. Romeu, I. Prieto, "Behaviour of insulin Langmuir monolayers at the air-water interface under various conditions", Thin Solid Films, vol. 516, pp. 8873-8879, 2008.
 K.Ariga, T. Kunitake, "Molecular Recognition at Air-Water and Related Interfaces: Complementary Hydrogen Bonding and Multisite Interaction", Acc. Chem. Res, vol. 31, pp. 371-378, 1998.
 J. M. Valderramaa, A. P. Gunning, M. J. Ridout, P. J. Wilde, V. J. Morris, "The effect of physiological conditions on the surface structure of proteins: Setting the scene for human digestion of emulsions", European Physical Journal E, vol. 30, pp. 165-174, 2009.
 K. Ariga, J. P. Hill, M. V. Lee, A. Vinu, R. Charvet, S. Acharya, "Challenges and Breakthroughs in Recent Research on Self-Assembly", Science and Technology in Advanced Materials, Vol. 9, pp. 14109- 14206, 2008.
 S. Joy, P. Pal, M. Mahato, G. B. Talapatra, S. Goswami, "Tailor Made Synthesis of Amphiphillic Azoaromatics via Regioselective C-N Bond Fusion: Comparative Studies of Surface Properties of the Two Positional Isomers and Cobalt Complexes", Dalton Transaction, vol. 39 pp. 2775- 2784, 2010.
 P. Pal, T. Kamilya, M. Mahato, G. B. Talapatra, "The formation of pepsin monomolecular layer by the Langmuir Blodgett tfilm deposition technique", Colloids Surfuce B: Biointerface, vol. 73, pp. 122-131, 2009.
 M. Mahato, P. Pal, B. Tah, M. Ghosh, G.B. Talapatra, "Study of Silver- Nanoparticle Hemoglobin Interaction and composite formations", Colloids Surfuce B: Biointerface, vol. 88, pp. 141-149, 2011.
 M. Mahato, P. Pal, T. Kamilya, B. Tah, R. Sarkar, G. B. Talapatra, "Fibrillation of Egg White Ovalbumin: A Pathway Via Biomineralization", Journal of Physical Chemistry B, vol. 115, pp. 4259-4265, 2011.
 T. Kamilya, P. Pal, M. Mahato,; G. B. Talapatra, "Effect of Salt on the Formation of Alcohol-Dehydrogenease Monolayer: A Study by the Langmuir-Blodgett Technique", Journal of Physical Chemistry B, vol. 113, pp. 5128-5135, 2009.
 K. H. Wang, M. J. Syu, C. H. Chang, Lee, Y.L., "Headgroup Effects of Template Monolayers on the Adsorption Behavior and Conformation of Glucose Oxidase Adsorbed at Air/Liquid Interfaces", Langmuir, vol. 27, pp. 7595-7602, 2011.
 P. L. Wang, G. O. Udeani, T. P. Johnston, "Inhibition of granulocytecolony- stimulating factor (G-CSF) adsorption to polyvinyl-chloride using a nonionic surfactant", International Journal of Pharmaceutical, vol. 114, p.p 177-184, 1995.
 H.Thurow, K.Geisen, "Stabilisation of dissolved proteins against denaturation at hydrophobic interfaces" Diabetologia, vol. 27, pp. 212- 218, 1984.
 W. D. Lougheed, A. M. Albisser, H. M. Martindale, J. C. Chow, J. R. Clement, "Physical stability of insulin formulations", Diabetes, vol. 32, pp. 424-432, 1983.
 B. S. Chang, B. S. Kendrick, J. F.Carpenter," Surface-Induced Denaturation of Proteins During Freezing and Its Inhibition by Surfactants" Journal of Pharmaceutical, vol. 85, pp. 1325-1330, 1996.
 N. B. Bam, J. L. Cleland, J. Yang, M. C. Manning, J. F. Carpenter, R. F. Kelley, T. W. Randolph, "Tween protects recombinant human growth hormone against agitation-induced damage via hydrophobic interactions" Journal of Pharmaceutical ,vol. 87, pp. 1554-1559, 1998.
 L. Kreilgaard, L. S. Jones, T. W. Randolph, S. Frokjaer, J. M. Flink, M. C. Manning, J. F. Carpenter, "Effect of Tween 20 on freeze-thawingand agitation-induced aggregation of recombinant human factor XIII", Journal of Pharmaceutical Science, vol. 87, pp. 1597-1603, 1998.
 S. H. Mollmann, U. Elofsson, J. T. Bukrinsky, S. Frokjaer, "Displacement of Adsorbed Insulin by Tween 80 Monitored Using Total Internal Reflection Fluorescence and Ellipsometry" Pharmaceutical Research vol. 22, pp. 1931- 1941, 2005, Nov.
 F. Sarmiento, G. Prieto, M. N. Jones, "Thermodynamic studies on the interaction of n-alkyl sulfates with insulin in aqueous solution", Journal of Chemical Society. Faraday Transaction, vol. 88, pp. 1003-1007, 1992.
 C. Aiello, P. Andreozzi, C. L. Mesa, G. Risuleo, "Biological activity of SDS-CTAB cat-anionic vesicles in cultured cells and assessment of their cytotoxicity ending in apoptosis" Colloids and Surfaces B: Biointerfaces, vol. 78, pp. 149-154, 2010.
 B. Tah, P. Pal, M. Mahato, G. B. Talapatra, "Aggregation behavior of SDS/CTAB cationic surfactant mixture in aqueous solution and at the air-water interface", Journal of Physical Chemistry B, vol. 115, pp. 8493, 2011.
 K. Shi, F. Cui, Y. Yu, L. Zhang, A. Tao, D. Cun, "Preparation and characterization of a novel insulin-phospholipid complex", Asian Journal of Pharmaceutical Sciences, vol. 1, pp. 168-174, 2006
 J. Lin, M. Nakagawa, K. Uchiyama, T. Hobo, "Determination of critical micelle concentration of SDS in formamide by capillary electrophoresis", Chromatographia, vol. 50 pp. 739-744, 1999.
 A. Cifuentes, J. L. Bernal, J. C. Diez-Masa, "Determination of Critical Micelle Concentration Values Using Capillary Electrophoresis Instrumentation", Analytical Chemistry, vol.69, pp. 4271-4274, 1997.
 A. Khan, E. Marques, "Catanionic Surfactants. In: Specialists Surfactants", I. D. Robb, Ed.; Blackie Academic and Professional: Londo,pp. 37-76, 1997.
 M. Mahato, P. Pal, T. Kamilya, R. Sarkar, A. Chaudhuri, G. B. Talapatra, "Influence of KCl on the Interfacial Activity and Conformation of Hemoglobin Studied by Langmuir Blodgett Technique", Physical Chemistry Chemical Physics, vol. 12, pp. 12997, 2010.
 P. Pal, M. Mahato, T. Kamilya, G. B. Talapatra," Interaction of glucose with hemoglobin: A study in aqueous solution and at air-water interface using Langmuir Blodgett technique", Physical Chemistry Chemical Physics, vol. 13 pp. 9385-9396, 2011.
 T. Kamilya, P. Pal, G. B. Talapatra, "Interaction of Ovalbumin with Phospholipids Langmuir-Blodgett Film", Journal of Physica. Chemistry B, vol. 111, pp. 1199-1205, 2007.
 G. Narsimhan, F. Uraizee, "Kinetics of adsorption of globular proteins at an air-water interface" Biotechnol. Progress, vol. 8, pp. 187-196, 1992.
 X. Yin, J. Goudriaan, E. A. Lantinga, J. Vos and H. J. Spiertz, "A Flexible Sigmoid Function of Determinate Growth", Annals of Botany, vol. 91, pp. 361-371, 2003.
 R. Mahfoud, N. Garmy, M. Maresca, N. Yahi, A. Puigserver and J. Fantini, "Identification of a Common Sphingolipid-binding Domain in Alzheimer, Prion, and HIV-1 Proteins" The Journal of Biological Chemistry, vol. 277, pp. 11292-11296, 2002.
 G. Prieto, M. del. Rio, M. I. Paz Andrade, F. Sarmiento, M. N. Jones, "Interaction between sodium n-undecyl sulfate and insulin", International Journal of Biological Macromol, vol. 15, pp. 343-345, 1993.
 K. K. Andersen, P. Westh, D. E. Otzen, "Global Study of Myoglobin- Surfactant Interactions", Langmuir, vol. 24, pp. 399-407, 2008.
 RCSB Protein Data Bank.
 M. N. Su'arez, N. V Romeu, P. D. Latka, "Behavior of insulin- sphingomyelin mixed Langmuir monolayers spread at the air-water interface", Colloids Surface A, vol. 321 pp. 189-195, 2008.
 K. Balashev, Tz. Ivanova, K. Mircheva, I. Panaiotov, "Savinase proteolysis of insulin Langmuir monolayers studied by surface pressure and surface potential measurements accompanied by atomic force microscopy (AFM) imaging.", Journal of Colloid and Interface Science, vol. 360, pp. 654-661, 2011.
 J. Llopis, D.V. Rebollo, "Influence of temperature on insulin monolayers.", Archives of Biochemistry and Biophysics, vol. 88, pp. 142-149, 1960.
 M. N. Su├írez, N. V. Romeu, I. Prieto, "Behaviour of insulin Langmuir monolayers at the air-water interface under various conditions" Thin Solid Films, vol. 516, pp. 8873-8879, 2008.
 S.Y. Venyaminov, N.N. Kalnin, "Quantitative IR spectrophotometry of peptide compounds in water (H2O) solutions. I. Spectral parameters of amino acid residue absorption bands", Biopolymers, vol. 30, pp. 1243- 1257, 1990.
 C. Letizia, P. Andreozzi, A. Scipioni, C. La Mesa, A. Bonincontro, E. Spigone, "Protein Binding onto Surfactant-Based Synthetic Vesicles"Journal of Physical Chemistry. B, vol. 111, pp. 898-908, 2007.
 J. Wei, Y. Z. Lin, J. M. Zhou, C. L. Tsou, "FTIR studies of secondary structures of bovine insulin and its derivatives" Biochimica et Biophysica Acta, vol. 1080, pp. 29-33, 1991.