%0 Journal Article %A Yan Ren and Fei Guo and Jun Qiao and Shengwei Hu and Hui Zhang and Yuanzhi Wang and Pengyan Wang and Jinliang Sheng and Xinli Gu and Xiaojun Liu and Chuangfu Chen %D 2013 %J International Journal of Biotechnology and Bioengineering %B World Academy of Science, Engineering and Technology %I Open Science Index 75, 2013 %T Identification of Binding Proteins That Interact with BVDV E2 Protein in Bovine Trophoblast Cell %U https://publications.waset.org/pdf/7100 %V 75 %X Bovine viral diarrhea virus (BVDV) can cause lifelong persistent infection. One reason for the phenomena is attributed to BVDV infection to placenta tissue. However the mechanisms that BVDV invades into placenta tissue remain unclear. To clarify the molecular mechanisms, we investigated the possible means that BVDV entered into bovine trophoblast cells (TPC). Yeast two-hybrid system was used to identify proteins extracted from TPC, which interact with BVDV envelope glycoprotein E2. A PGbkt7-E2 yeast expression vector and TPC cDNA library were constructed. Through two rounds of screening, three positive clones were identified. Sequencing analysis indicated that all the three positive clones encoded the same protein clathrin. Physical interaction between clathrin and BVDV E2 protein was further confirmed by coimmunoprecipitation experiments. This result suggested that the clathrin might play a critical role in the process of BVDV entry into placenta tissue and might be a novel antiviral target for preventing BVDV infection. %P 239 - 242