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Colorectal Cancer Screening by a CEACAM-6 Immunosensor
Abstract:The biomarker for colorectal cancer (CRC) is CEACAM-6 antigen (C6AG). Therefore, this study aims to develop a novel, simple and low-cost CEACAM-6 antigen immumosensor (C6AG-IMS), based on electrical impedance measurement, for precise determination of C6AG. A low-cost screen-printed graphite electrode was constructed and used as the sensor, with CEACAM-6 antibody (C6AB) immobilized on it. The procedures of sensor fabrication and antibody immobilization are simple and low-cost. Measurement of the electrical impedance at a definite frequency ranges (0.43 – 1.26 MHz) showed that the C6AG-IMS has an excellent linear (r2>0.9) response range (8.125 – 65 pg/mL), covering the normal physiological and pathological ranges of blood C6AG levels. Also, the C6AG-IMS has excellent reliability and validity, with the intraclass correlation coefficient being 0.97. In conclusion, a novel, simple, low-cost and reliable C6AG-IMS was designed and developed, being able to accurately determine blood C6AG levels in the range of pathological and normal physiological regions. The C6AG-IMS can provide a point-of-care and immediate screening results to the user at home.
Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1332970Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 1300
 GLOBOCAN 2008IARC Section of Cancer Information, WORLD - Most frequent cancers: both sexes, Available at: http://globocan.iarc.fr/factsheets/populations/factsheet.asp?uno=900, Last accessed: 23rd March 2012.
 Department of Health, Executive Yuan, R.O.C.(TAIWAN), Health Statistics in Taiwan, 2010, Available at: http://www.doh.gov.tw/CHT2006/DisplayStatisticFile.aspx?d=81298&s=1, Last accessed: 23rd March 2012.
 IT IS, Available at: http://www.itis.org.tw/index.jsp, Last accessed: 23rd March 2012.
 http://homepage.vghtpe.gov.tw/~crs/disease/colcancer2.htm#1, Last accessed: 23rd March 2012.
 P. Jantscheff, L. Terracciano, A. Lowy and et al., Expression of CEACAM6 in resectable colorectal cancer: a factor of independent prognostic significance, J Clin Oncol, vol. 21, pp. 3638-3646, 2003.
 L. Portney and M. Watkins, Foundations of Clinical Research: applications to practice, 2nd ed., New Jersey: Prentice Hall Health, 2000.
 L. Portney and M. Watkins, Foundations of Clinical Research: applications to practice, 2nd ed., New Jersey: Prentice Hall Health, 2000, pp565.