%0 Journal Article %A Rungsinee Phongpradist and Sawitree Chiampanichayakul and Singkome Tima and Teruna J. Siahaan and Cory J. Berkland and Songyot Anuchapreeda and Chadarat Ampasavate %D 2012 %J International Journal of Pharmacological and Pharmaceutical Sciences %B World Academy of Science, Engineering and Technology %I Open Science Index 66, 2012 %T Potential cIBR-Conjugated PLGA Nanoparticles for Selective Targeting to Leukemic Cells %U https://publications.waset.org/pdf/6057 %V 66 %X The expression of LFA-1 diverges from the physiological condition, thus active targeting carrier can provide the benefits from difference into LFA-1 expression in various conditions. Here, the selectivity of cIBR-conjugated nanoparticles (cIBR-NPs), in terms of uptake, was investigated using PBMCs, Mixed PBMCMolt- 3 cells and Molt-3 cells. The expressions of LFA-1 on Molt-3 cells, from flow cytometry and Western blot, possessed the highest level whereas PBMCs showed the lowest level. The kinetic uptake profiles of cIBR-NPs were obtained by flow cytometry, which the degree of cellular uptake presented a similar trend with the level of LFA-1 indicating the influence of LFA-1 expression on the cellular uptake of cIBR-NPs. The conformation of LFA-1 had a slight effect on the cellular uptake of cIBR-NPs. Overall we demonstrated that cIBR-NPs enhanced cellular uptake and improved the selectivity of drug carriers to LFA-1 on the leukemia cells, which related with the order of LFA-1 expression. %P 234 - 242