Mohamed Hafez and Georg Hausner
Characterization of the O.ulmS952 IntronA Potential Molecular Marker to Distinguish Between Ophiostoma Ulmi and Ophiostoma NovoUlmi Subsp. Americana
715 - 723
2011
5
11
International Journal of Biotechnology and Bioengineering
https://publications.waset.org/pdf/5301
https://publications.waset.org/vol/59
World Academy of Science, Engineering and Technology
The full length mitochondrial small subunit ribosomal
(mtrns) gene has been characterized for Ophiostoma novoulmi
subspecies americana. The gene was also characterized for
Ophiostoma ulmi and a group II intron was noted in the mtrns gene
of O. ulmi. The insertion in the mtrns gene is at position S952 and it
is a group IIB1 intron that encodes a double motif LAGLIDADG
homing endonuclease from an open reading frame located within a
loop of domain III. Secondary structure models for the mtrns RNA
of O. novoulmi subsp. americana and O. ulmi were generated to
place the intron within the context of the ribosomal RNA. The in vivo
splicing of the O.ulmS952 group II intron was confirmed with
reverse transcriptionPCR. A survey of 182 strains of Dutch Elm
Diseases causing agents showed that the mS952 intron was absent in
what is considered to be the more aggressive species O. novoulmi
but present in strains of the less aggressive O. ulmi. This observation
suggests that the O.ulmS952 intron can be used as a PCRbased
molecular marker to discriminate between O. ulmi and O. novoulmi
subsp. americana.
Open Science Index 59, 2011