Leukocytes Count and Lymphocyte Proliferation of Dinitrochlorobenzene Sensitized Rat Supplemented with Fermented Goat Milk
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Leukocytes Count and Lymphocyte Proliferation of Dinitrochlorobenzene Sensitized Rat Supplemented with Fermented Goat Milk

Authors: Nurliyani, Eni Harmayani, Marsetyawan HNE Soesatyo

Abstract:

Goat milk has an hypoallergenic effects, and allergic diseases related to abnormal of intestinal flora. Probiotic microorganisms do exert an activity on the immune system in the skin of the individual.The purpose of this study are to determine the number of leukocyte and lymphocyte proliferation in rat supplemented with fermented goat milk (acidophilus milk and kefir) and sensitized with dinitrochlorobenzene (DNCB). Female Wistar rats 6-8 weeks olds were divided into 3 treatment groups. The first group supplemented goat milk kefir, second group acidophilus goat milk, and third group as control. During 28-day experiment, on day 15 rat sensitized with allergen DNCB on the dorsal of the body, and on day 24 was challenged with DNCB on the ear. Sampling of blood and tissue of intestinal Peyer'patch (PP) were performed on day 14 (before DNCB sensitized) and on day 28 (after DNCB sensitized). The results showed the number of neutrophils in rats supplemented with acidophilus milk was higher (P<0.05) in after DNCB sensitized than before, but the lymphocyte count was lower. The number of monocytes, eosinophils, and basophils before and after DNCB sensitized have the same average for all treatments of milk fermented and control. Fermented goat milk (kefir and acidophilus milk) did not affect on rat PP lymphocyte proliferation culture supernatant, whereas the rat that had been DNCB sensitized showed higher in proliferative response to PHA mitogen (P <0.05) than before sensitized. In conclusion, supplementation of acidophilus goat milk with a dose of 2.0 ml / head / day on DNCB sensitized rat, can increase the number of neutrophils that play a role in innate immunity, however it was not able to increase lymphocyte proliferation that related to adaptive immunity.

Keywords: Leukocytes, Lymphocyte proliferation, Kefir, Acidophilus milk, Dinitrochlorobenzene

Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1328562

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References:


[1] B. Wang, H. Fujisawa, L. Zhuang, I. Freed, B.G. Howell, S. Shahid, G.M. Shivji, T.W. Mak, and D.N. Sauder, "CD4+ Th1 and CD8+ type 1 cytotoxic T cells both play a crucial role in the full development of contact hypersensitivity", The Journal of Immunology, 165, pp.6783- 6790, 2000.
[2] E. Marschan, " Immunological effects of probiotic bacteria in prevention and treatment of allergic diseases in children", Publication of the National Public Health Institute, Department of Viral Diseases and Immunology, National Public Health Institute Helsinki, Findland and Hospital for Children and Adolescents. University of Helsinki, Findland, 2007
[3] T. Kullisaar, E. Songisepp, M. Mikelsaar, K. Zilmer, T. Vihalemm, and M. Zilmer, "Antioxidative probiotic fermented goat-s milk decreases oxidative stress-mediated atherogenicity in human subjects", British Journal of Nutrition, vol. 90, pp. 449-456, 2003.
[4] M. Baur, F. Couzy, and A. Gueniche, "Use of probiotic lactic acid bacteria for balancing the skin-s immune system", United States Patent, No.: US 7,547,527 B2, 2009
[5] C.Traidl, F. Judgert, T. Krieg, H. Merk, and N. Hunzelmann. 1999. Inhibition of allergic contact dermatitis to DNCB but not to oxazolone in interleukine-4-deficient mice. The journal of Investigative Dermatology, vol. 112, no.4, pp. 476-482, 1999.
[6] P.G. Reeves, F.H. Neilsen and G.C. Fahey, JR. "AIN-93 Purified Diets for Laboratory Rodents: FinalReport of the American Institute of Nutrition Ad Hoc Writing Committee on the Formulation of the AIN- 76A Rodent Diet", J. Nutr., vol.123. pp. 1939-1951, 1993
[7] S. Oties, and O. Cangindi 2003. "Kefir: A probiotic dairy-composition, nutritional and therapeutic aspects", Pakistan Journal of Nutrition, vol. 2, no.2. pp. 54-59, 2003
[8] BJB Wood, "Microbiology of Fermented Foods", Elsevier Applied Science Publishers, 1985, vol 1.
[9] Benjamin, M.M. 1981. Outline of Veterinary Chemical Pathology. 3 rd Edition. The Iowa State University Press, Iowa. USA.
[10] J. Chen, C-Pei Ng, D.K. Rowlands, P-Hui Xu, J-Y Gao, Y-Wa Chung, H-C Chan, "Interaction between enteric epithelial cells and Peyer-s patch lymphocytes in responses to Shigella lipopoysaccharide : Effect on nitric oxide and IL-6 release", World J Gastroenterol, vol. 12, no.24, pp. 3895-3900, June 2006.
[11] M..A. Wijayanti, "The Role of Macrophages in Immunity against Malaria Infection: A Study of Phagocytic and Oxygen Radical Intermediates Secretion Ability on Peritoneal Macrophage by In Vitro Immunized and Non Immunized Mice". Thesis. Public Health Studies Program, Department of Health Sciences. Universitas Gadjah Mada, Yogyakarta, Indonesia, 1996.
[12] F.I. Aboderin, and V.O. Oyetaya. 2006, "Haematological studies of rats fed different doses of probiotic Lactobacillus plantarum, isolated from fermenting corn slurry", Pakistan Journal of Nutrition, vol. 5, no.2, pp. 102-105.
[13] G.A. Azeredo, T.L.M. Stamford, E.L. de Souza, F.F. Veras, E.R de Almeida, and J M de Araujo, "In vivo assessment of possible probiotic properties of Zymomonas mobilis in a Wistar rat model", Electronic Journal of Biotechnology, vol. 13, no.2, pp. 1-7, 2010.
[14] D.G. Cameron, and G.M. Watson. The blood counts of adult albino rat. www.bloodjournal.org, 2010.
[15] Anonymous, Neutrophils - Anti-microbial Functions of the neutrophils. http://blood.ygoy.com/neutrophils-anti-microbial-functions-of-theneutrophils/, 2010
[16] A. Latifynia, M. Vojgani, M.J. Gharagozlou, R. Sharifian, " Neutrophil function (innate immunity) during ramadan", J Ayub Med Coll Abbottabad, vol. 21, no. 4, pp. 111-115, 2009.
[17] S.K. Ramaiah and H. Jaeschke, " Role of neutrophil in the pathogenesis of acut inflammatory liver injury", Toxicologic Pathology, vol. 35, pp. 757-766, 2007.
[18] Z. Effendi, "The role of leukocyte as anti-inflammatory in the body", Department of Histology, Faculty of Medicine, Universitas Sumatera Utara, 2003
[19] J.G. Wheeler, S.J. Shema, M.L. Bogle, M.A. Shirrel, A.W. Burks, A. Pittler and R.M.Helm," Immune and clinical impact of Lactobacillus acidophilus on asthma", Annals of Allergy, Asthma & Immunology, vol.79, no. 3, pp. 229-233, 1997.
[20] Anonymous, "Acute inflammation", http://courses.washington.edu/conj/inflammation/acuteinflam.htm 2 011
[21] B.M.Ogilvie, M.H. Patricia, and M.E. Rose, "Nippostrongylus brasiliensis: Peripheral blood leucocyte response of rats , with special reference to basophils", Experimental Parasitology vol. 46, pp. 20-30, 1978
[22] Y. Li, L. Lixin, R. Wadley, S.W. Reddel, J.C. Qi, C. Archis, A. Collins, E. Clark, M. Cooley, S. Kouts, H.M. Naif, M. Alali, A. Cunningham, G.W. Wong, R.L. Stevens, and S.A. Krilis, "Mast cells/basophils in peripheral blood of allergic individuals who are HIV-1 susceptible due to their surface expression of CD4 and the chemokine receptors CCR3, CCR5, and CXCR4", Blood, vol. 97, no.11, pp. 3484-3490, 2001.
[23] I. Roitt, J. Brostoff, and D. Male, " Immunology", 3rd ed. Mosby- Year Book Europe Ltd. London, 1993, pp. 19.1-19.13.
[24] H.S. Gill, K.J. Rutherfurd, J. Prasad, P.K.Gopal, " Enhancement of natural and acquired immunity by Lactobacillus rhamnosus (HN001), Lactobacillus acidophilus (HN017) and Bifidobacterium lactis (HN019)", Br J Nutr. vol. 83, no.2, pp.167-176, 2000
[25] H.V.L.N. Swamy, T.K. Smith, E.J. MacDonald, N.A. Karrow, B. Woodward, and H.J. Boermans, "Effect of feeding a blend of grains naturally contaminated with Fusarium mycotoxin on growth and immunological measurements of starter pigs, and the efficacy of polymeric glucomannan mycotoxin adsorbent", J. Anim. Sci, vol . 81.pp. 2792-2803.