Nguyen Anh Dzung and Nguyen Thi Ngoc Hà and Dang Thi Hong Van and Nguyen Thi Lan Phuong and Nguyen Thi
Nhu Quynh and Dinh Minh Hiep and Le Van Hiep
Chitosan Nanoparticle as a Novel Delivery System for AH1n1 Influenza Vaccine Safe Property and Immunogenicity in Mice
915 - 922
2011
5
12
International Journal of Biotechnology and Bioengineering
https://publications.waset.org/pdf/5089
https://publications.waset.org/vol/60
World Academy of Science, Engineering and Technology
The aims of this paper are to study the efficacy of
chitosan nanoparticles in stimulating specific antibody against
AH1N1 influenza antigen in mice. Chitosan nanoparticles (CSN)
were characterized by TEM. The results showed that the average size
of CSN was from 80nm to 106nm. The efficacy of AH1N1 influenza
vaccine loaded on the surface of CSN showed that loading efficiency
of AH1N1 influenza antigen on CSN was from 93.75 to 100. Safe
property of the vaccine were tested. In 10 days post vaccination,
group of CSN 30 kDa and 300 kDa loaded AH1N1 influenza antigen
were the rate of immune response on mice to be 100 (99) higher
than Al(OH)3 and other adjuvant. 100 mice in the experiment of all
groups had immune response in 20 days post vaccination. The results
also showed that HI titer of the group using CSN 300 kDa as an
adjuvant increased significantly up to 3971 HIU, over threefold
higher than the Al(OH)3 adjuvant, chitosan (CS), and one hundredfold
than the AH1N1 antigen only. Stability of the vaccine
formulation was investigated.
Open Science Index 60, 2011