@article{(Open Science Index):https://publications.waset.org/pdf/4754,
	  title     = {Evaluation of Newly Developed Dot-ELISA Test for Identification of Naja-naja sumantrana and Calloselasma rhodostoma Venom Antigens},
	  author    = {A.S. Sikarwar and  S. Ambu and  T .H. Wong},
	  country	= {},
	  institution	= {},
	  abstract     = {Snake bite cases in Malaysia most often involve the
species Naja-naja and Calloselasma rhodostoma. In keeping with the
need for a rapid snake venom detection kit in a clinical setting, plate
and dot-ELISA test for the venoms of Naja-naja sumatrana,
Calloselasma rhodostoma and the cobra venom fraction V antigen
was developed. Polyclonal antibodies were raised and further used to
prepare the reagents for the dot-ELISA test kit which was tested in
mice, rabbit and virtual human models. The newly developed dot-
ELISA kit was able to detect a minimum venom concentration of
244ng/ml with cross reactivity of one antibody type. The dot-ELISA
system was sensitive and specific for all three snake venom types in
all tested animal models. The lowest minimum venom concentration
detectable was in the rabbit model, 244ng/ml of the cobra venom
fraction V antigen. The highest minimum venom concentration was
in mice, 1953ng/ml against a multitude of venoms. The developed
dot-ELISA system for the detection of three snake venom types was
successful with a sensitivity of 95.8% and specificity of 97.9%.},
	    journal   = {International Journal of Medical and Health Sciences},
	  volume    = {6},
	  number    = {8},
	  year      = {2012},
	  pages     = {394 - 396},
	  ee        = {https://publications.waset.org/pdf/4754},
	  url   	= {https://publications.waset.org/vol/68},
	  bibsource = {https://publications.waset.org/},
	  issn  	= {eISSN: 1307-6892},
	  publisher = {World Academy of Science, Engineering and Technology},
	  index 	= {Open Science Index 68, 2012},
	}