%0 Journal Article %A Aree Thongpukdee and Ekasit Nisayan and Chockpisit Thepsithar %D 2013 %J International Journal of Bioengineering and Life Sciences %B World Academy of Science, Engineering and Technology %I Open Science Index 78, 2013 %T Multiple Shoot Formation of Paphiopedilum 'Delrosi' %U https://publications.waset.org/pdf/3966 %V 78 %X Shoots, with three leaves, of Paphiopedilum 'Delrosi' were used as explants for multiple shoot induction. Modified Hyponex medium was supplemented with thidiazuron (TDZ), N6- benzyladenine (BA) or kinetin (Kn) alone and in combinations with 2,4-dichlorophenoxyacetic acid (2,4-D). All explants were cultured for 15 weeks. It was found that TDZ alone at the concentration of 0.45μM or in combination with 4.52μM 2,4-D and 8.88μM BA in combination with 13.56μM 2,4-D promoted multiple shoots. The highest shoot sprouting efficiencies (80.0, 90.0 and 80.0%) and new shoot numbers (1.5, 1.3 and 1.1) were obtained, respectively. Fresh weight, height, numbers of leaf and root of new shoots and initial explants were discussed. %P 399 - 402