Commenced in January 2007
Frequency: Monthly
Edition: International
Paper Count: 30458
Antibiotic Resistance Profile of Bacterial Isolates from Animal Farming Aquatic Environments and Meats in a Peri-Urban Community in South Korea

Authors: Hyunjin Rho, Bongjin Shin, Okbok Lee, Yu-Hyun Choi, Jiyoung Lee, Jaerang Rho

Abstract:

The increasing usage of antibiotics in the animal farming industry is an emerging worldwide problem contributing to the development of antibiotic resistance. The purpose of this work was to investigate the prevalence and antibiotic resistance profile of bacterial isolates collected from aquatic environments and meats in a peri-urban community in Daejeon, Korea. In an antibacterial susceptibility test, the bacterial isolates showed a high incidence of resistance (~ 26.04 %) to cefazolin, tetracycline, gentamycin, norfloxacin, erythromycin and vancomycin. The results from a test for multiple antibiotic resistance indicated that the isolates were displaying an approximately 5-fold increase in the incidence of multiple antibiotic resistance to combinations of two different antibiotics compared to combinations of three or more antibiotics. Most of the isolates showed multi-antibiotic resistance, and the resistance patterns were similar among the sampling groups. Sequencing data analysis of 16S rRNA showed that most of the resistant isolates appeared to be dominated by the classes Betaproteobacteria and Gammaproteobacteria in the phylum Proteobacteria.

Keywords: Antibiotics, Antibiotic Resistance, Antimicrobial resistance, Multi-resistance

Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1331245

Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 1829

References:


[1] Alanis, A.J. 2005. Resistance to antibiotics: are we in the post-antibiotic era? Arch Med Res 36:697-705.
[2] Levy, S.B., and Marshall, B. 2004. Antibacterial resistance worldwide: causes, challenges and responses. Nat Med 10:S122-129.
[3] Mathew, A.G., Cissell, R., and Liamthong, S. 2007. Antibiotic resistance in bacteria associated with food animals: a United States perspective of livestock production. Foodborne Pathog Dis 4:115-133.
[4] Shryock, T.R., and Richwine, A. 2010. The interface between veterinary and human antibiotic use. Ann N Y Acad Sci 1213:92-105.
[5] Ding, C., and He, J. 2010. Effect of antibiotics in the environment on microbial populations. Appl Microbiol Biotechnol 87:925-941.
[6] Davies, J. 1994. Inactivation of antibiotics and the dissemination of resistance genes. Science 264:375-382.
[7] Davison, J. 1999. Genetic exchange between bacteria in the environment. Plasmid 42:73-91.
[8] Alekshun, M.N., and Levy, S.B. 2007. Molecular mechanisms of antibacterial multidrug resistance. Cell 128:1037-1050.
[9] Kimiran-Erdem, A., Arslan, E.O., Sanli Yurudu, N.O., Zeybek, Z., Dogruoz, N., and Cotuk, A. 2007. Isolation and identification of enterococci from seawater samples: assessment of their resistance to antibiotics and heavy metals. Environ Monit Assess 125:219-228.
[10] Servais, P., and Passerat, J. 2009. Antimicrobial resistance of fecal bacteria in waters of the Seine river watershed (France). Sci Total Environ 408:365-372.
[11] Witte, W. 2000. Ecological impact of antibiotic use in animals on different complex microflora: environment. Int J Antimicrob Agents 14:321-325.
[12] Kilonzo-Nthenge, A., Nahashon, S.N., Chen, F., and Adefope, N. 2008. Prevalence and antimicrobial resistance of pathogenic bacteria in chicken and guinea fowl. Poult Sci 87:1841-1848.
[13] Bauer, A.W., Kirby, W.M., Sherris, J.C., and Turck, M. 1966. Antibiotic susceptibility testing by a standardized single disk method. Am J Clin Pathol 45:493-496.
[14] Reboucas, R.H., de Sousa, O.V., Lima, A.S., Vasconcelos, F.R., de Carvalho, P.B., and Vieira, R.H. 2011. Antimicrobial resistance profile of Vibrio species isolated from marine shrimp farming environments (Litopenaeus vannamei) at Ceara, Brazil. Environ Res 111:21-24.
[15] Cho, S.H., Han, J.H., Ko, H.Y., and Kim, S.B. 2008. Streptacidiphilus anmyonensis sp. nov., Streptacidiphilus rugosus sp. nov. and Streptacidiphilus melanogenes sp. nov., acidophilic actinobacteria isolated from Pinus soils. Int J Syst Evol Microbiol 58:1566-1570.
[16] Hur, J., Choi, Y.Y., Park, J.H., Jeon, B.W., Lee, H.S., Kim, A.R., and Lee, J.H. 2011. Antimicrobial resistance, virulence-associated genes, and pulsed-field gel electrophoresis profiles of Salmonella enterica subsp. enterica serovar Typhimurium isolated from piglets with diarrhea in Korea. Can J Vet Res 75:49-56.
[17] Kang, M.S., Kim, A., Jung, B.Y., Her, M., Jeong, W., Cho, Y.M., Oh, J.Y., Lee, Y.J., Kwon, J.H., and Kwon, Y.K. 2010. Characterization of antimicrobial resistance of recent Salmonella enterica serovar Gallinarum isolates from chickens in South Korea. Avian Pathol 39:201-205.
[18] Nam, H.M., Lim, S.K., Moon, J.S., Kang, H.M., Kim, J.M., Jang, K.C., Kang, M.I., Joo, Y.S., and Jung, S.C. 2010. Antimicrobial resistance of enterococci isolated from mastitic bovine milk samples in Korea. Zoonoses Public Health 57:e59-64.
[19] Oh, E.G., Son, K.T., Yu, H., Lee, T.S., Lee, H.J., Shin, S., Kwon, J.Y., Park, K., and Kim, J. 2011. Antimicrobial resistance of Vibrio parahaemolyticus and Vibrio alginolyticus strains isolated from farmed fish in Korea from 2005 through 2007. J Food Prot 74:380-386.
[20] Unno, T., Han, D., Jang, J., Lee, S.N., Kim, J.H., Ko, G., Kim, B.G., Ahn, J.H., Kanaly, R.A., Sadowsky, M.J., et al. 2010. High diversity and abundance of antibiotic-resistant Escherichia coli isolated from humans and farm animal hosts in Jeonnam Province, South Korea. Sci Total Environ 408:3499-3506.
[21] Lim, S.K., Lee, H.S., Nam, H.M., Cho, Y.S., Kim, J.M., Song, S.W., Park, Y.H., and Jung, S.C. 2007. Antimicrobial resistance observed in Escherichia coli strains isolated from fecal samples of cattle and pigs in Korea during 2003-2004. Int J Food Microbiol 116:283-286.
[22] Li, D., Yu, T., Zhang, Y., Yang, M., Li, Z., Liu, M., and Qi, R. 2010. Antibiotic resistance characteristics of environmental bacteria from an oxytetracycline production wastewater treatment plant and the receiving river. Appl Environ Microbiol 76:3444-3451.
[23] Joseph, S.W., Hayes, J.R., English, L.L., Carr, L.E., and Wagner, D.D. 2001. Implications of multiple antimicrobial-resistant enterococci associated with the poultry environment. Food Addit Contam 18:1118-1123.
[24] Hammerum, A.M., Lester, C.H., and Heuer, O.E. 2010. Antimicrobial-resistant enterococci in animals and meat: a human health hazard? Foodborne Pathog Dis 7:1137-1146.
[25] Heuer, O.E., Hammerum, A.M., Collignon, P., and Wegener, H.C. 2006. Human health hazard from antimicrobial-resistant enterococci in animals and food. Clin Infect Dis 43:911-916.
[26] Butaye, P., Devriese, L.A., Goossens, H., Ieven, M., and Haesebrouck, F. 1999. Enterococci with acquired vancomycin resistance in pigs and chickens of different age groups. Antimicrob Agents Chemother 43:365-366.
[27] Butaye, P., Devriese, L.A., and Haesebrouck, F. 1999. Comparison of direct and enrichment methods for the selective isolation of vancomycin-resistant enterococci from feces of pigs and poultry. Microb Drug Resist 5:131-134.
[28] Bates, J. 1997. Epidemiology of vancomycin-resistant enterococci in the community and the relevance of farm animals to human infection. J Hosp Infect 37:89-101.
[29] Bates, J., Jordens, J.Z., and Griffiths, D.T. 1994. Farm animals as a putative reservoir for vancomycin-resistant enterococcal infection in man. J Antimicrob Chemother 34:507-514.
[30] Knapp, C.W., Engemann, C.A., Hanson, M.L., Keen, P.L., Hall, K.J., and Graham, D.W. 2008. Indirect evidence of transposon-mediated selection of antibiotic resistance genes in aquatic systems at low-level oxytetracycline exposures. Environ Sci Technol 42:5348-5353.
[31] Fan, C., Lee, P.K., Ng, W.J., Alvarez-Cohen, L., Brodie, E.L., Andersen, G.L., and He, J. 2009. Influence of trace erythromycin and eryhthromycin-H2O on carbon and nutrients removal and on resistance selection in sequencing batch reactors (SBRs). Appl Microbiol Biotechnol 85:185-195.
[32] Esiobu, N., Armenta, L., and Ike, J. 2002. Antibiotic resistance in soil and water environments. Int J Environ Health Res 12:133-144.
[33] Biyela, P.T., Lin, J., and Bezuidenhout, C.C. 2004. The role of aquatic ecosystems as reservoirs of antibiotic resistant bacteria and antibiotic resistance genes. Water Sci Technol 50:45-50.
[34] Schwartz, T., Kohnen, W., Jansen, B., and Obst, U. 2003. Detection of antibiotic-resistant bacteria and their resistance genes in wastewater, surface water, and drinking water biofilms. FEMS Microbiol Ecol 43:325-335.