%0 Journal Article %A Jing Lei and Yoram Vodovotz and Timothy R. Billiar %D 2007 %J International Journal of Medical and Health Sciences %B World Academy of Science, Engineering and Technology %I Open Science Index 5, 2007 %T Endothelial Specificity of ICAM2, Flt-1, and Tie2 Promoters In Vitro and In Vivo %U https://publications.waset.org/pdf/3621 %V 5 %X To identify an endothelial cell-specific promoter suitable for vascular-specific targeting, we tested five promoters in vitro--Tie2SE, Tie2LE, ICAM2, Flt-1 and vWF--for promoter activity and specificity in endothelial cells, smooth muscle cells and non-vascular resident cells as well as tissues. These promoters, except for vWF, exhibited good endothelial activity and specificity in vitro. In a syngenic heart transplantation model, the ICAM2 promoter was variably functional in coronary endothelial cells of donor hearts. Thus, the ICAM2, Flt-1, Tie2SE and Tie2LE promoters hold promise for endothelial-specific targeting, but in vitro expression may not predict in vivo expression. %P 250 - 254