WASET
	%0 Journal Article
	%A Jing Lei and  Yoram Vodovotz and  Timothy R. Billiar
	%D 2007
	%J International Journal of Medical and Health Sciences
	%B World Academy of Science, Engineering and Technology
	%I Open Science Index 5, 2007
	%T Endothelial Specificity of ICAM2, Flt-1, and Tie2 Promoters In Vitro and In Vivo
	%U https://publications.waset.org/pdf/3621
	%V 5
	%X To identify an endothelial cell-specific promoter suitable for vascular-specific targeting, we tested five promoters in vitro--Tie2SE, Tie2LE, ICAM2, Flt-1 and vWF--for promoter activity and specificity in endothelial cells, smooth muscle cells and non-vascular resident cells as well as tissues. These promoters, except for vWF, exhibited good endothelial activity and specificity in vitro. In a syngenic heart transplantation model, the ICAM2 promoter was variably functional in coronary endothelial cells of donor hearts. Thus, the ICAM2, Flt-1, Tie2SE and Tie2LE promoters hold promise for endothelial-specific targeting, but in vitro expression may not predict in vivo expression.

	%P 250 - 254