O. Grinchuk and E. Motakis and V. Kuznetsov
Identification of Complex Senseantisense Gene&039;s Module on 17q11.2 Associated with Breast Cancer Aggressiveness and Patient&039;s Survival
273 - 282
2009
3
10
International Journal of Biomedical and Biological Engineering
https://publications.waset.org/pdf/3188
https://publications.waset.org/vol/34
World Academy of Science, Engineering and Technology
Senseantisense gene pair (SAGP) is a pair of two oppositely transcribed genes sharing a common region on a chromosome. In the mammalian genomes, SAGPs can be organized in more complex senseantisense gene architectures (CSAGA) in which at least one gene could share loci with two or more antisense partners. Many dozens of CSAGAs can be found in the human genome. However, CSAGAs have not been systematically identified and characterized in context of their role in human diseases including cancers. In this work we characterize the structuralfunctional properties of a cluster of 5 genes –TMEM97, IFT20, TNFAIP1, POLDIP2 and TMEM199, termed TNFAIP1 POLDIP2 module. This cluster is organized as CSAGA in cytoband 17q11.2. Affymetrix U133A&B expression data of two large cohorts (410 atients, in total) of breast cancer patients and patient survival data were used. For the both studied cohorts, we demonstrate (i) strong and reproducible transcriptional coregulatory patterns of genes of TNFAIP1POLDIP2 module in breast cancer cell subtypes and (ii) significant associations of TNFAIP1POLDIP2 CSAGA with amplification of the CSAGA region in breast cancer, (ii) cancer aggressiveness (e.g. genetic grades) and (iv) disease free patients survival. Moreover, gene pairs of this module demonstrate strong synergetic effect in the prognosis of time of breast cancer relapse. We suggest that TNFAIP1 POLDIP2 cluster can be considered as a novel type of structuralfunctional gene modules in the human genome.
Open Science Index 34, 2009