%0 Journal Article
	%A Chun-Yen Sung and  Chung-Yao Yang and  Tzu-Chun Liao and  Wen-Shiang Chen and  Chao-Min Cheng and  J. Andrew Yeh
	%D 2013
	%J International Journal of Biomedical and Biological Engineering
	%B World Academy of Science, Engineering and Technology
	%I Open Science Index 74, 2013
	%T Neuroblasts Micropatterning on Nanostructural Modified Chitosan Membranes
	%U https://publications.waset.org/pdf/2943
	%V 74
	%X The study describes chitosan membrane platform
modified with nanostructure pattern which using nanotechnology to
fabricate. The cell-substrate interaction between neuro-2a neuroblasts
cell lines and chitosan membrane (flat, nanostructure and
nanostructure pattern types) was investigated. The adhered
morphology of neuro-2a cells depends on the topography of chitosan
surface. We have found that neuro-2a showed different morphogenesis
when cells adhered on flat and nanostructure chitosan membrane. The
cell projected area of neuro-2a on flat chitosan membrane is larger
than on nanostructure chitosan membrane. In addition, neuro-2a cells
preferred to adhere on flat chitosan surface region than on
nanostructure chitosan membrane to immobilize and differentiation.
The experiment suggests surface topography can be used as a critical
mechanism to isolate group of neuro-2a to a particular rectangle area
on chitosan membrane. Our finding will provide a platform to take
patch clamp to record electrophysiological behavior about neurons in
vitro in the future.
	%P 91 - 94