@article{(Open Science Index):https://publications.waset.org/pdf/2943, title = {Neuroblasts Micropatterning on Nanostructural Modified Chitosan Membranes}, author = {Chun-Yen Sung and Chung-Yao Yang and Tzu-Chun Liao and Wen-Shiang Chen and Chao-Min Cheng and J. Andrew Yeh}, country = {}, institution = {}, abstract = {The study describes chitosan membrane platform modified with nanostructure pattern which using nanotechnology to fabricate. The cell-substrate interaction between neuro-2a neuroblasts cell lines and chitosan membrane (flat, nanostructure and nanostructure pattern types) was investigated. The adhered morphology of neuro-2a cells depends on the topography of chitosan surface. We have found that neuro-2a showed different morphogenesis when cells adhered on flat and nanostructure chitosan membrane. The cell projected area of neuro-2a on flat chitosan membrane is larger than on nanostructure chitosan membrane. In addition, neuro-2a cells preferred to adhere on flat chitosan surface region than on nanostructure chitosan membrane to immobilize and differentiation. The experiment suggests surface topography can be used as a critical mechanism to isolate group of neuro-2a to a particular rectangle area on chitosan membrane. Our finding will provide a platform to take patch clamp to record electrophysiological behavior about neurons in vitro in the future.}, journal = {International Journal of Biomedical and Biological Engineering}, volume = {7}, number = {2}, year = {2013}, pages = {91 - 94}, ee = {https://publications.waset.org/pdf/2943}, url = {https://publications.waset.org/vol/74}, bibsource = {https://publications.waset.org/}, issn = {eISSN: 1307-6892}, publisher = {World Academy of Science, Engineering and Technology}, index = {Open Science Index 74, 2013}, }