@article{(Open Science Index):https://publications.waset.org/pdf/2644, title = {Molecular Epidemiology and Genotyping of Bovine Viral Diarrhea Virus in Xinjiang Uygur Autonomous Region of China}, author = {Yan Ren and Jun Qiao and Xianxia Liu and Pengyan Wang and Qiang Fu and Huijun Shi and Fei Guo and Yuanzhi Wang and Hui Zhang and Jinliang Sheng and Xinli Gu and Xiao-Jun Liu and Chuangfu Chen}, country = {}, institution = {}, abstract = {As part of national epidemiological survey on bovine viral diarrhea virus (BVDV), a total of 274 dejecta samples were collected from 14 cattle farms in 8 areas of Xinjiang Uygur Autonomous Region in northwestern China. Total RNA was extracted from each sample, and 5--untranslated region (UTR) of BVDV genome was amplified by using two-step reverse transcriptase-polymerase chain reaction (RT-PCR). The PCR products were subsequently sequenced to study the genetic variations of BVDV in these areas. Among the 274 samples, 33 samples were found virus-positive. According to sequence analysis of the PCR products, the 33 samples could be arranged into 16 groups. All the sequences, however, were highly conserved with BVDV Osloss strains. The virus possessed theses sequences belonged to BVDV-1b subtype by phylogenetic analysis. Based on these data, we established a typing tree for BVDV in these areas. Our results suggested that BVDV-1b was a predominant subgenotype in northwestern China and no correlation between the genetic and geographical distances could be observed above the farm level.}, journal = {International Journal of Biotechnology and Bioengineering}, volume = {7}, number = {3}, year = {2013}, pages = {234 - 238}, ee = {https://publications.waset.org/pdf/2644}, url = {https://publications.waset.org/vol/75}, bibsource = {https://publications.waset.org/}, issn = {eISSN: 1307-6892}, publisher = {World Academy of Science, Engineering and Technology}, index = {Open Science Index 75, 2013}, }