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Construction of cDNALibrary and EST Analysis of Tenebriomolitorlarvae
Abstract:Tofurther advance research on immune-related genes from T. molitor, we constructed acDNA library and analyzed expressed sequence taq (EST) sequences from 1,056 clones. After removing vector sequence and quality checkingthrough thePhred program (trim_alt 0.05 (P-score>20), 1039 sequences were generated. The average length of insert was 792 bp. In addition, we identified 162 clusters, 167 contigs and 391 contigs after clustering and assembling process using a TGICL package. EST sequences were searchedagainst NCBI nr database by local BLAST (blastx, E
Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1056212Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 1193
 J. W. Park, et al., "A synthetic peptidoglycan fragment as a competitive inhibitor of the melanization cascade," J Biol Chem, vol. 281, pp. 7747-55, Mar 24 2006.
 K. B. Roh, et al., "Proteolytic cascade for the activation of the insect toll pathway induced by the fungal cell wall component," J Biol Chem, vol. 284, pp. 19474-81, Jul 17 2009.
 H. Steiner, et al., "Sequence and specificity of two antibacterial proteins involved in insect immunity," Nature, vol. 292, pp. 246-8, Jul 16 1981.
 D. Hultmark, et al., "Insect immunity. Attacins, a family of antibacterial proteins from Hyalophora cecropia," EMBO J, vol. 2, pp. 571-6, 1983.
 H. G. Boman, et al., "Insect immunity--a gene system for antibacterial proteins," Mem Inst Oswaldo Cruz, vol. 82 Suppl 3, pp. 115-24, 1987.
 J. Lambert, et al., "Insect immunity: isolation from immune blood of the dipteran Phormia terranovae of two insect antibacterial peptides with sequence homology to rabbit lung macrophage bactericidal peptides," Proc Natl Acad Sci U S A, vol. 86, pp. 262-6, Jan 1989.
 R. Jiang, et al., "Three pairs of protease-serpin complexes cooperatively regulate the insect innate immune responses," J Biol Chem, vol. 284, pp. 35652-8, Dec 18 2009.
 B. Ewing, et al., "Base-calling of automated sequencer traces using phred. I. Accuracy assessment," Genome Res, vol. 8, pp. 175-85, Mar 1998.
 B. Ewing and P. Green, "Base-calling of automated sequencer traces using phred. II. Error probabilities," Genome Res, vol. 8, pp. 186-94, Mar 1998.
 S. F. Altschul, et al., "Basic local alignment search tool," J Mol Biol, vol. 215, pp. 403-10, Oct 5 1990.
 R. L. Tatusov, et al., "The COG database: an updated version includes eukaryotes," BMC Bioinformatics, vol. 4, p. 41, Sep 11 2003.
 G. Pertea, et al., "TIGR Gene Indices clustering tools (TGICL): a software system for fast clustering of large EST datasets," Bioinformatics, vol. 19, pp. 651-2, Mar 22 2003.
 D. H. Kim, et al., "Internalization of tenecin 3 by a fungal cellular process is essential for its fungicidal effect on Candida albicans," Eur J Biochem, vol. 268, pp. 4449-58, Aug 2001.