TY - JFULL AU - Victor A. Naumenko and Yuriy A. Tyulenev and Alla A. Kushch PY - 2012/1/ TI - Herpes Simplex Virus Type I Infection of Mice Testis and Effect on Fertility T2 - International Journal of Medical and Health Sciences SP - 702 EP - 706 VL - 6 SN - 1307-6892 UR - https://publications.waset.org/pdf/1938 PU - World Academy of Science, Engineering and Technology NX - Open Science Index 72, 2012 N2 - The objective of current issue was to develop a model of testicular herpes simplex virus (HSV) type I infection for assessment of viral effect on fertility. 56 male mice were inoculated intraperitoneally with different concentrations of HSV on 8 day post partum. It was revealed that the optimal dose was 100 plaque forming units per mice as it provided testicular infection in 100% of survivors. HSV proteins were detected both in somatic and germ cells (spermatogonia, spermatocytes, spermatides). Although DNA load in testis was descending from 3 to 28 days post infection only 12.5% of infected males had offspring after mating with uninfected females comparing to 87.5% in control (p=0.012). These results are the first direct evidence for HSV impact in male sterility. Prepuberal mice appeared to be a suitable model for investigation of pathogenesis of virus-associated fertility disorders. ER -