Nile Red, an Alternative Fluorescence Method for Quantification of Neutral Lipids in Microalgae
According to biodiesel from microalgae is an attractive fuel for several reasons such as renewable, biodegradable and environmental friendly. Thus, this study, green microalgae Scenedesmus acutus PPNK1 isolated from natural water, was selected based on high growth rates, easy cultivation and high lipid content. The Nile red fluorescence method has been successfully applied to the determination of lipids in S. acutus PPNK1. The combination of the method to the lipid composition in algal cells showed the yellow fluorescence under fluorescent microscope. Interestingly, maximum cell numbers and biomass concentration were obtained at 5.44´107 cells/mL and 1.60 g/L when it was cultivated in BG-11 medium while in case of BG-11 with nitrogen deprivation (N 0.25 g/L), accumulated lipid content in cells (44.67%) was achieved that was higher than that found in case of BG-11 medium at about 2 times (22.63%).
Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1087716Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 6601
 A. L. Ahmad, N. H. Mat Yasin, C. J. C. Derek,, and J. K. Lim. 2011. Microalgae as a sustainable energy source for biodiesel production: A review. Renewable and Sustainable Energy Reviews. 15,584- 593.
 T. M. Mata, A. A. Martins and N. S. Caetano 2010. Microalgae for biodiesel production and other applications. Renewable and Sustainable Energy Reviews. 14, 217-232.
 C. Y. Chen, K. L. Yeh, R. Aisyah, D. J. Lee, and J. S. Chang 2011. Cultivation, photobioreactor design and harvesting of microalgae for biodiesel production: A critical review Bioresource Technology. 102, 71-81.
 W. Chen, M. Sommerfeld, M., and Q. Hu. 2011. Microwave-assisted Nile red method for in vivo quantification of neutral lipids in microalgae. Bioresource Technology. 102, 135-141.
 S. H. Ho, W. M. Chen and J. S. Chang. 2010. Scenedesmus obliquus CNW-N as a potential candidate for CO2 mitigation and biodiesel production. Bioresource Technology. 101, 8725-8730.
 Q. Lin. and J. Lin. 2011. Effects of nitrogen source and concentration on biomass and oil production of a Scenedesmus rubescens like microalga. Bioresource Technology. 102, 1615-1621.
 J. Msanne, D. Xu, A. R. Konda, J. A. Casas-Mollano, T. Awada, E. B. Cahoon and H. Cerutt. 2012. Metabolic and gene expression changes triggered by nitrogen deprivation in the photoautotrophically grown microalgae Chlamydomonas reinhardtii and Coccomyxa sp. C-169. Phytochemistry. 75, 50-59.
 S. White, A. Anandraj, and F. Bux. 2011. PAM fluorometry as a tool to assess microalgal nutrient stress and monitor cellular neutral lipids. Bioresource Technology. 102, 1675-1682.
 D. Yan, Y. LU, Y. F. Chen, and Q. Wu. 2011. Waste molasses alone displaces glucose-based medium for microalgal fermentation towards cost-saving biodiesel production. Bioresource Technology.102, 6487- 6493.
 T. Govender, L. Ramanna, I. Rawat and F. Bux. 2012. BODIPY staining, an alternative to the Nile Red fluorescence method for the evaluation of intracellular lipids in microalgae. Bioresource Technology. 114, 507-511.
 E. Bertozzini, L. Galluzzi, A. Penna, and M. Magnani, 2011. Application of the standard addition method for the absolute quantification of neutral lipids in microalgae using Nile red. Journal of Microbiological Methods. 87, 17-23.
 G. H. Huang, G. Chen and F. Chen. 2009. Rapid screening method for lipid production in alga based on Nile red fluorescence. Biomass and Bioenergy. 33, 1386-1392.
 Y. Y. Pan, S. T. Wang, L. T. Chuang, Y. W. Chang, and C. N. Nathan Chen, 2011. Isolation of thermo-tolerant and high lipid content green microalgae: Oil accumulation is predominantly controlled by photosystem efficiency during stress treatments in Desmodesmus. Bioresource Technology. 102, 10510-10517.
 F. Han, J. Huang, Y. Li, W. Wang, J. Wang, J. Fan, and G. Shen. 2012. Enhancement of microalgal biomass and lipid productivities by a model of photoautotrophic culture with heterotrophic cells as seed. Bioresource Technology.118, 431-437.
 A. Converti, A. A. Casazza, E. Y. Ortiz, P. Perego, and M. D. Borghi. 2009. Effect of temperature and nitrogen concentration on the growth and lipid content of Nannochloropsis oculata and Chlorella vulgaris for biodiesel production. Chemical Engineering and Processing, 48, 1146- 1151.