Screening and Identification of Microorganisms – Potential Producers of Arachidonic Acid

Authors: A. V. Goncharova, T. A. Karpenyuk, Y. S. Tsurkan, R. U. Beisembaeva, A. M. Kalbaeva, T. D. Mukasheva, L. V. Ignatova

Abstract:

Microorganisms isolated from water and soil of Kazakhstan to identify potential high-effective producers of the arachidonic acid, exhibiting a wide range of physiological activity and having practical applications were screened. Based on the results of two independent tests (the test on the sensitivity of the growth processes of microorganisms to acetylsalicylic acid - an irreversible inhibitor of PGH-synthase involved in the metabolism of arachidonic acid and its derivatives, the test for inhibition of peroxidase activity of membrane-bounding fraction of PGH - synthase by acetylsalicylic acid) were selected microbial cultures which are potential highproducer of arachidonic acid. They are characterized by a stable strong growth in the laboratory conditions. Identification of microorganism cultures based on morphological, physiological, biochemical and molecular genetic characteristics was performed.

Keywords: Arachidonic acid, aspirin-sensitive culture, bacteria, producers, screening.

Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1087332

References:

[1] A. R. Brash, “Arachidonic acid as a bioactive molecule” J. of Clinical Investigation, vol.107, no. 11, pp. 1339-1345, 2001.
[2] M. G. Sergeeva, A. T. Varfolomeeva, The cascade of arachidonic acid. Moscow: Public education, 2006, 256 p.
[3] S. H. Mitmesser, C. L. Jensen, “Roles of long-chain polyunsaturated fatty acids in the term infant: developmental benefits”, Neonatal Network, vol. 26, no. 4, pp. 229-234, 2007.
[4] G. Bernardi, New comprehensive biochemistry: Biochemistry of lipids, lipoproteins and membranes, Amsterdam: Elsevier, 1996, pp. 141–152.
[5] E. G. Deduhina, T. I. Chistyakova, M. B. Vainshtein, “Biosynthesis of arachidonic acid by Micromycetes” (review), Applied biochemistry and microbiology, vol. 47, no. 2, pp. 125-134, 2011.
[6] K. Higashiyama, S. Fujikawa, E. Y. Park, S. Shimizu, “Production of Arachidonic Acid by Mortierella Fungi”, Biotechnology and Bioprocess Engineering, vol. 7, pp. 252-262, 2002.
[7] Edited by A. N. Netrusova М. Workshop on Microbiology, Academia, 2005, pp. 448- 597.
[8] E. Z. Tepper, V. K. Shilnikova, Workshop on Microbiology, Мoscow: Drofa, 2004, 256 p.
[9] Edited by N.S Yegorov, Guide to practical training in Microbiology: Textbook, 3rd ed., Moscow: Moscow State University Press, 1995, 224p.
[10] V. K. Eroshin, E. G. Dedyukhina, T. I. Chistyakova, V. P. Zhelifonova, C. P. Kurtzman, R. J. Bothast, “Arachidonic-acid production by species of Mortierella”, World Journal of Microbiology & Biotechnology, vol. 12, no. 1, pp. 91-96, 1996.
[11] J. G. Holt, Noel. R. Krieg, Peter H. A. Sneath, James T. Staley, Stanley T. Williams, Bergey's Manual of Determinative Bacteriology”, 9-th. Ed, vol.1-2, 1997.
[12] I. N. Scvortcova, Identification of soil bacteria of the Bacillus, Part 1, Мoscow: MSU, 1983, 43 p.
[13] O. A. Nesterenko, E. N. Kvasnikov, T. M. Nogina, Nocardio and corinesimilar bacteria, Kiev, 1985, 331 p.
[14] K. Wilson, “Preparation of genomic DNA from bacteria”, Current Protocols in Molecular Biology, (F. M. Ausubel, R. Brent, R. E. Kingston, D. D. Moore, J. G. Seidman, J. A. Smith, et al.), New York: Wiley, 1987, 650 p.
[15] R. A. Clayton, G. Sutton, P. S. Hinkle, Jr. C. Bult, C. Fields, “Intraspecific variation in small-subunit rRNA sequences in GenBank: why single sequences may not adequately represent prokaryotic taxa” International Journal of Systematic Bacteriology, vol. 45, pp. 595-599, 1995.
[16] E. Werle, C. Schneider, M. Renner, M. Völker, W. Fiehn, “Convenient single-step, one tube purification of PCR products for direct sequencing”, Nucleic Acids Research, vol. 22, pp. 4354-4355, 1994.
[17] H. Maciel, C. Gouvêa, M. Toyama, M. Smolka, S. Marangoni, G. Pastore, “Еxtraction, purification and biochemical characterization of a peroxidase from Copaifera langsdorffii leaves”, Química Nova, vol. 30, no. 5, pp. 1067-1071, 2007.
[18] O. H. Lowry, N. J. Rosebrough, A. L. Farr, R. J. Randall, “Protein measurement with Folin phenol reagent”, Journal of Biological Chemistry, vol.193, no 1, pp. 265-275,1951.
[19] P. I. Terentyev, N. S. Rostova, Workshop on Biometrics, Leningrad: SP. U., 1977, 234 p.
[20] J. R. Vane, R. M. Botting, “The mechanism of action of aspirin”, Thrombosis Research, no. 110, pp. 255–258, 2003.