%0 Journal Article
	%A Pavel Pořízka and  David Prochazka and  Karel Novotný and  Ota Samek and  ZdeněkPilát and  Klára Procházková and  and
Jozef Kaiser
	%D 2012
	%J International Journal of Bioengineering and Life Sciences
	%B World Academy of Science, Engineering and Technology
	%I Open Science Index 72, 2012
	%T Utilization of Laser-Ablation Based Analytical Methods for Obtaining Complete Chemical Information of Algae
	%U https://publications.waset.org/pdf/15981
	%V 72
	%X Themain goal of this article is to find efficient
methods for elemental and molecular analysis of living
microorganisms (algae) under defined environmental conditions and
cultivation processes. The overall knowledge of chemical
composition is obtained utilizing laser-based techniques, Laser-
Induced Breakdown Spectroscopy (LIBS) for acquiring information
about elemental composition and Raman Spectroscopy for gaining
molecular information, respectively. Algal cells were suspended in
liquid media and characterized using their spectra. Results obtained
employing LIBS and Raman Spectroscopy techniques will help to
elucidate algae biology (nutrition dynamics depending on cultivation
conditions) and to identify algal strains, which have the potential for
applications in metal-ion absorption (bioremediation) and biofuel
industry. Moreover, bioremediation can be readily combined with
production of 3rd generation biofuels. In order to use algae for
efficient fuel production, the optimal cultivation parameters have to
be determinedleading to high production of oil in selected
cellswithout significant inhibition of the photosynthetic activity and
the culture growth rate, e.g. it is necessary to distinguish conditions
for algal strain containing high amount of higher unsaturated fatty
acids. Measurements employing LIBS and Raman Spectroscopy were
utilized in order to give information about alga Trachydiscusminutus
with emphasis on the amount of the lipid content inside the algal cell
and the ability of algae to withdraw nutrients from its environment
and bioremediation (elemental composition), respectively. This
article can serve as the reference for further efforts in describing
complete chemical composition of algal samples employing laserablation
techniques.
	%P 1155 - 1159