@article{(Open Science Index):https://publications.waset.org/pdf/15807,
	  title     = {High Efficiency, Selectivity against Cancer Cell Line of Purified L-Asparaginase from Pathogenic Escherichia coli},
	  author    = {Hazim Saadoon Aljewari and  Mohammed Ibraheem Nader and  Abdul Hussain M. Alfaisal and  NatthidaWeerapreeyakul and  Sahapat},
	  country	= {},
	  institution	= {},
	  abstract     = {L-asparaginase was extracted from pathogenic
Escherichia coli which was isolated from urinary tract infection
patients. L-asparaginase was purified 96-fold by ultrafiltration, ion
exchange and gel filtration giving 39.19% yield with final specific
activity of 178.57 IU/mg. L-asparaginase showed 138,356±1,000
Dalton molecular weight with 31024±100 Dalton molecular mass.
Kinetic properties of enzyme resulting 1.25×10-5 mM Km and
2.5×10-3 M/min Vmax. L-asparaginase showed a maximum activity
at pH 7.5 when incubated at 37 ºC for 30 min and illustrated its full
activity (100%) after 15 min incubation at 20-37 ºC, while 70% of its
activity was lost when incubated at 60 ºC. L-asparaginase showed
cytotoxicity to U937 cell line with IC50 0.5±0.19 IU/ml, and
selectivity index (SI=7.6) about 8 time higher selectivity over the
lymphocyte cells. Therefore, the local pathogenic E. coli strains may
be used as a source of high yield of L-asparaginase to produce anti
cancer agent with high selectivity.},
	    journal   = {International Journal of Pharmacological and Pharmaceutical Sciences},
	  volume    = {4},
	  number    = {5},
	  year      = {2010},
	  pages     = {199 - 204},
	  ee        = {https://publications.waset.org/pdf/15807},
	  url   	= {https://publications.waset.org/vol/41},
	  bibsource = {https://publications.waset.org/},
	  issn  	= {eISSN: 1307-6892},
	  publisher = {World Academy of Science, Engineering and Technology},
	  index 	= {Open Science Index 41, 2010},
	}