Physiological and Biochemical Responses to Drought Stress of Chickpea Genotypes
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Physiological and Biochemical Responses to Drought Stress of Chickpea Genotypes

Authors: E. Ceyhan, A. Kahraman, M. Önder, M.K. Ateş, S. Karadaş, R. Topak, M.A. Avcı

Abstract:

The experimental design was 4 x 5 factorial with three replications in fully controlled research greenhouse in Department of Soil Sciences and Plant Nutrition, Faculty of Agriculture, University of Selcuk in the year of 2009. Determination of tolerant chickpea genotypes to drought was made in the research. Additionally, sophisticated effects of drought on plant growth and development, biochemical and physical properties or physical defense mechanisms were presented. According to the results, the primary genotypes were Ilgın YP (0.0063 g/gh) for leaf water capacity, 22235 70.44(%) for relative water content, 22159 (82.47%) for real water content, 22159 (5.03 mg/l) for chlorophyll a+b, Ilgın YP (125.89 nmol H2O2.dak-1/ mg protein-1) for peroxidase, Yunak YP (769.67 unit/ mg protein-1) for superoxide dismutase, Seydişehir YP (16.74 μg.TA-1) for proline, Gökçe (80.01 nmol H2O2.dak-1/ mg protein-1) for catalase. Consequently, all the genotypes increased their enzyme activity depending on the increasing of drought stress consider with the effects of drought stress on leaf enzyme activity. Chickpea genotypes are increasing enzyme activity against to drought stress.

Keywords: Chickpea, drought, enzyme, tolerance to drought

Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1083561

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References:


[1] O. Önder, M., Ceyhan, E. and Kahraman, A., 2011. Effects of Agricultural Practices on Environment. Biology, Environment and Chemistry (ICBEC 2011), Volume 24, Page 28-32, December 28-30, Dubai, UAE.
[2] Kahraman, A., Önder, M. and Ceyhan, E., 2011. Biodiversity and biosecurity in Turkey. Biology, Environment and Chemistry (ICBEC 2011), Volume 24, Page 33-37, December 28-30, Dubai, UAE.
[3] Ceyhan, E., Kahraman, A. and Önder, M., 2012. The effects of environment on plant products. International Journal of Bioscience, Biochemistry and Bioinformatics, Volume 2, no 1, Page 48-51.
[4] Kalefetoğlu, T., 2006, Nohut (Cicer arietinum L.) çeşit ve hatlarının kuraklık stresine karşı dayanıklılığının karakterizasyonu. Haccetepe Universiy, Institute of Life Sciences, Master Thesis. pp: 142.
[5] Clarke, J.M. and McGaig, T.N.1982, Excised-leaf water retention capability as an indicator of drought. J. Plant Sci., 62: 571-578.
[6] Farrant, J.M., 2000, A comparison of mechanisms of desiccation tolerance among three angiosperm resurrection plant species. Plant Eco., 151, 29-39.
[7] Lichtenthaler, H.K., 1987. Chlorophylls and carotenoids: pigments of photosynthetic biomembranes. Meth Enzymol, 148: 350-382.
[8] Kumar, K.B. and P.A. Khan, 1982. Peroxidase and polyphenol oxidase in excised ragi (Eleusine coracana cv. PR 202) leaves during senescence. Indian J. Experimental Botany, 20: 412-416.
[9] Beauchamp, C. ve Fridovich, I., 1971, Superoxide Dismutase: Improved Assays and Applicable to Acrylamide Gels. Analytical Biochemistry. 44: 276-287 p.
[10] Bates, L.S., Waldren, R.P., Teare, I.D., 1973, Rapid determination of free proline for water-stress studies. Plant Soil, 39, 205- 207.
[11] Bergmeyer, H.U., 1970. Methods of Enzymatic Analysis. Verlag Chemie, Weinheim/Bergstr, German.
[12] Düzgüneş O., Kesici T., Kavuncu, O. ve Gürbüz, F. 1987, Araştırma ve Deneme Metodları (İstatiksel Metodlar-II). Ankara Univ.Agricultural Fac. Press No:1021, Class Book Serial No:295. Ankara.
[13] Fu, J. and Huang, B., 2001, Involvement of antioxidants and lipid peroxidation in the adaptation of two cool-season grasses to localized drought stress. Environ. Exp. Bot., 45, 105-114.
[14] Egert, M. and Tevini, M. 2002, Influence of drought on some physiological parameters symptomatic for oxidative stress in leaves of chives (Allium schoenoprasum). Environ. Exp. Bot., 48. 43-49.
[15] Liu, F. and St├╝tzel, H. 2002, Leaf water relations of vegetable amaranth (Amaranthus spp.) in response to soil drying. Eur. J. Agron., 16, 137- 150.
[16] Tambussi, E. A., Casadesus, J., Munné-Bosch, S., Araus, J. L., 2002, Photoprotection in water-stressed plants of durum wheat (Triticum turgidum var. durum): changes in chlorophyll fluorescence spectral signature and photosynthetic pigments. Funct. Plant Biol., 29, 35-44.
[17] Anyia, A.O. and Herzog, H., 2004. Genotypic Variability in Drought Performance and Recovery in Cowpea under Controlled Environment. J. Agronomy & Crop Science, 190, 151ÔÇö159.
[18] Lucero, M. E., Mueller, W., Hubstenberger, J., Phillips, G. C., O-Connell, M. A., 1999. Tolerance to nitrogenous explosives and metabolism of TNT by cell suspensions of Datura innoxia. In Vitro Cell Div. Biol. Plant, 35: 480-486.
[19] Costa França, M.G., Pham-Thi, C.A.T., Pimentel, R.O.P., Rossiello, Y., Fodil, Z., Laffray, D., 2000, Differences in growth and water relations among Phaseolus vulgaris cultivars in response to induced drought stress. Environ. Exp. Bot., 43, 227-237.
[20] Soltani, A., Khooie, F. R., Ghassemi-Golezani K. and Moghaddam, M. 2000. Thresholds for chickpea leaf expansion and transpiration response to soil water deficit. Field Crops Res., 68 (3), 205-210.
[21] Munné-Bosch, S., Jubany-Mari, T., Alegre, L., 2001, Drought-induced senescence is characterised by a loss of antioxidant defences in chloroplasts. Plant. Cell Environ., 24, 1319-1327.
[22] Srivalli, B., Sharma, G. and Khanna-Chopra, R., 2003. Antioxidative defense system in an upland rice cultivar subjected to increasing intensity of water stress followed by recovery. Physiologia Plantarum, 119: 503-512.
[23] Jung, S., 2004, Variation in antioxidant metabolism of young and mature leaves of Arabidopsis thaliana subjected to drought. Plant Sci., 166, 459- 466.
[24] Lazaridou, M. and Koutroubas, S.D., 2004. Drought effect on water use efficiency of berseem clover at various growth stages . Proceeding for the 4th International Crop Science Congress, Brisbane, Australia, 26 September-1 October 2004.
[25] Jiang, H.F. and Ren, X.P., 2004, The effect on SOD activity and protein content in groundnut leaves by drought stress. AAS, 30, 169- 174.
[26] Asada, K. and Takahashi, M., 1987, Production and scavenging of active oxygen radicals in photosynthesis. Photoinhibition. Kyle. D.J. (ed.). Elsevier. pp. 227-297.
[27] Ramachandra Reddy, A., Viswanatha Chaitanya, K., Vivekanandan, M., 2004, Drought-induced responses of photosynthesis and antioxidant metabolism in higher plants. J. Plant Physiol.,161, 1189-1202.
[28] HongBo, S., ZongSuo, L., MingAn, S., 2005, Changes of anti-oxidative enzymes and MDA content under soil water deficits among 10 wheat (Triticum aestivum L.) genotypes at maturation stage. Colloids and Surfaces B: Biointerfaces, 45, 7-13.
[29] Türkan, İ., Bor. M., Özdemir, F., Koca, H. 2005, Differential responses of lipid peroxidation and antioxidants in the leaves of drought-tolerant P. acutifolius Gray and drought-sensitive P. vulgaris L. subjected to polyethylene glycol mediated water stress. Plant Sci., 168, 223-231.
[30] Ge, T., Sui, F., Bai, L., Lu, Y., Zhou, G., 2006, Effects of water stress on the protective enzyme activities and lipid peroxidation in roots and leaves of summer maize. ASC, 5(4), 291-298.
[31] Shao L, Young LT, Wang JF. Chronic treatment with mood stabilizers lithium and valproate prevents excitotoxicity by inhibiting oxidative stress in rat cerebral cortical cells. Biol Psychiatry 2005;58:879-84.
[32] T─▒p─▒rdamaz, R. ve ├çak─▒rlar. H. 1990, Bu─ƒday (Triticum aestivum L.) bitkisinin T├╝rkiye-de yeti┼ƒtirilen iki ├ºe┼ƒidinde tuz ve su stresinin oransal su kapsam─▒ prolin ve betain de─ƒi┼ƒimine etkisi. DO─×A-Tr. J. of Biology. 14 ( 2), 125-148.
[33] Hsu, S.Y., Hsu, Y.T., Kao, C.H., 2003, The effect of polyethylene glycol on proline accumulation in rice leaves. Biol. Plant., 46, 73-78.
[34] Kavi Kishore, P.B., Sangam, S., Amrutha, R.N., Laxmi, P.S., Naidu, K.R., Rao, K.R.S.S., Rao, S., Reddy, K.J., Theriappan, P., Sreenivasulu, N., 2005, Regulation of proline biosynthesis, degradation, uptake and transport in higher plants: its implications in plant growth and abiotic stress tolerance. Curr. Sci., 88, 424-438.
[35] Tan, Y., Liang, Z., Shao, H., Du, F., 2006, Effect of water deficits on the activity of anti-oxidative enzymes and osmoregulation among three different genotypes of Radix astragali at seeding stage. Colloid. Surface. B., 49, 59-64.