Identification Characterization and Production of Phytase from Endophytic Fungi

Authors: Yetti Marlida , Rina Delfita , Neni Gusmanizar, Gita Ciptaan

Abstract:

Phytases are acid phosphatase enzymes, which efficiently cleave phosphate moieties from phytic acid, thereby generating myo-inositol and inorganic phosphate. Thirty four isolates of endophytic fungi to produce of phytases were isolated from leaf, stem and root fragments of soybean. Screening of 34 isolates of endophytic fungi identified the phytases produced by Rhizoctonia sp. and Fusarium verticillioides . The phytase production were the best induced by phytic acid and rice bran compared the others inducer in submerged fermentation medium used. The phytase produced by both Rhizoctonia sp. and F. verticillioides have pH optimum at 4.0 and 5.0 respectively. The characterization of phytase from Fusarium verticillioides showed that temperature optimum was 500C and stability until 600C, the pH optimum 5.0 and pH stability was 2.5 – 6.0, and substrate specificity were rice bran>soybean meal>corn> coconut cake, respectively.

Keywords: endophytic fungus, phytase, soybean, Rhizoctoniasp., Fusarium verticillioides,

Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1081023

References:

[1] J. Pallauf, and G. Rimbach. Nutritional Significance of Phytic Acid and Phytase. Arch. Anim. Nutr. . 1996; 50: 301-319.
[2] J. A., Martin, R. A. Murphy and R. F. F. Power. Purrification and Physico- Chemical Characterisation of Genetically Modifed Phytases Expressed in Aspergillus awamori. Bioresource Technology 2005. pp 97.
[3] L., W. Cao, Wang., C. Yang., Y. Yang., J. Diana., A. Yakupitiyage., Z. Luo And D. Li.. Application of Microbial Phytase in Fish Feed. J. Enzyme and Microbial Technology. 2007 40: 497-507.
[4] Z., H. Liu, Wang., Xiu-E Wang., H. Xu., D. Gao., G. Zhang,. P. Chen And D. Liu. Effect of Wheat Perling on Flour Phytase Activity, Phytic Acid, Iron and Zinc Content. Food Sci and Technology. 2007.
[5] N. Takizawa.. Utilization of Recombinan Phytase on the Avoidance of Water Pollution by Phosporus of Excretory Organ of Liverstock. Seitbutsu-kakagu. , 1998, pp 76.
[6] R Greiner,., Haller, E., Konietzny, U. and K. D. Jany.. Purification and Characterization of A Phytase From Klebsiella Terrigena. Arch. Biochem. Biophys. 1997, 341: 201-206.
[7] V. K. Powar, and V. Jagannathan.. Purfication and Properties of Phytate- Specific Phosphatase from Bacillus subtilis. J. Bacteriol. 1982, 151: 1102-1108.
[8] R,Greiner, Konietzny, U. and Jany, K. D.. Purification and Characterization of Two Phytases From Escherichia coli. Arch. Biochem. Biophys. 1993, 303: 107-113.
[9] S. J Yoon,., Y. J. Choi., H. K. Min., K. K Cho., J. W. Kim., S. C. Lee and Y. H.. Jung. Isolation and Identification of Phytase-Producing Bacterium, Enterobacter sp. 4, and Enzymatic Properties of Phytase Enzyme. Enzyme Microbiol. Technol. 1996., 18: 449-454.
[10] V, Shah, and Parekh, L. J.. Phytase From Klebsiella sp. No. Pg-2: Purification and Properties. Indian J. Biochem. Biophys. 1990, 27: 98- 102.
[11] S. J, Howson,. and R. J. Davis.. Production of Phytate-Hydrolysing Enzyme by Fungi. J. Enzyme Microbiol. Technol. 1983, 5: 377-382.
[12] C., L. Quan, Zhang., Y. Wang and Y. Ohta.. Prpduction of Phytase in Low Phosphate Medium by a Novel Yeast Candida krusei. Journal of Bioscience and Bioenginering. 2001, 9 (2): 154-160.
[13] R. A Samson, and E. S. van Reenen-Hoekstra.. Intoduction to Food- Borne Fungi. 1988, Centraalbureau voor Schimmelcultures. Wageningen.
[14] H. L ,Barnett, and B. B, Hunter.. Ilustrated Genera of Imperfect Fungi. Third Edition. 1972, Burgess Publishing Company. USA.
[15] T. W Kim, and X. G. Lei. 2005. An Improved Method for a Rapid Determination of Phytase activity in animal feed. J. Anim. Sci. 83: 1062- 1067.
[16] McKee, Trudy,.. Biochemistry: An Intoduction. Secend Edition. 1999, McGraw-Hill. Amerika.
[17] M ,Yetti.. Isolation and Purification of Raw Starch Degrading Enzyme from Acremonium endophytic Fungus and Its Aplication for Glucosa Production. Dissertation of Doctoral at University Putra Malaysia. Malaysia. 2001.
[18] M, Yetti., Saari N., Radu S and Bakar F.A.. Raw starch degrading enzyme from newly isolated strains of endophytic fungi. World Journal Microbiology and Biotechnology 2000, 16.575-578.
[19] A., Boyce and Walsh.,G.. Purification and characterization of an phosphatase with phytase activity from Mucor heimalis Wehmer. Journal of Biotechnology 2007, 132. 82-87.