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Langmuir–Blodgett Films of Polyaniline for Efficient Detection of Uric Acid

Authors: Kashima Arora, Monika Tomar, Vinay Gupta

Abstract:

Langmuir–Blodgett (LB) films of polyaniline (PANI) grown onto ITO coated glass substrates were utilized for the fabrication of Uric acid biosensor for efficient detection of uric acid by immobilizing Uricase via EDC–NHS coupling. The modified electrodes were characterized by atomic force microscopy (AFM). The response characteristics after immobilization of uricase were studied using cyclic voltammetry and electrochemical impedance spectroscopy techniques. The uricase/PANI/ITO/glass bioelectrode studied by CV and EIS techniques revealed detection of uric acid in a wide range of 0.05 mM to 1.0 mM, covering the physiological range in blood. A low Michaelis–Menten constant (Km) of 0.21 mM indicates the higher affinity of immobilized Uricase towards its analyte (uric acid). The fabricated uric acid biosensor based on PANI LB films exhibits excellent sensitivity of 0.21 mA/mM with a response time of 4 s, good reproducibility, long shelf life (8 weeks) and high selectivity.

Keywords: Uric acid; biosensor, PANI, Langmuir Blodgett films deposition.

Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1080886

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References:


[1] S.Dong et al.,"Electrochemical biosensors based on advanced bioimmobilization matrices", Mol. Biotechnol., vol. 82, pp. 303, 2002.
[2] T.Jiang et al., "Assembling Amperometric Biosensors for Clinical Diagnostics", Biosens. Bioelectron., vol. 20, pp. 1939, 2005.
[3] D.Borole et al.," Glucose oxidase electrodes of polyaniline, poly(otoluidine) and their copolymer as a biosensor: a comparative study", Poly. Adv. Tech., vol. 15, pp. 306, 2004.
[4] J.C.Vidal et al.," Comparison of biosensors based on entrapment of cholesterol oxidase and cholesterol esterase in electropolymerized films of polypyrrole and diaminonaphthalene derivatives for amperometric determination of cholesterol", Anal. Lett., vol. 35, pp.837, 2002.
[5] N.Osvaldo et al.,"Water at interfaces and its influence on the electrical properties of adsorbed films ", Braz. J. Phys., vol. 22, pp. 59, 1992.
[6] B.L.Hassler et al.," Recent advances self assembly monolayers based biomolecular electronic devices", Biosens. Bioelectron., vol. 21, pp. 2146, 2006.
[7] A.Ulman et al.," Formation and structure of self assembled monolayers", Chem. Rev., vol. 96, pp. 1533, 1996.
[8] J. M.Chovelon et al.," Nanostructuration and Nanoimaging of Biomolecules for Biosensors", Mater. Sci. Eng. C, vol. 22, pp. 79, 2002.
[9] H.Tsuji et al.," An amperometric glucose sensor with modified Langmuir-Blodgett films", Electroanal., vol. 9, pp. 161, 1997.
[10] Y.C. Luo et al.,"An amperometric uric acid biosensor based on modified Ir-C electrode", Biosens. Bioelectron., vol. 22, pp. 482-488, 2006.
[11] T. Ahuja et al., "An amperometric uric acid biosensor based on Bis
[sulfosuccinimidyl] suberate crosslinker/ 3-aminopropyl triethoxysilane surface modified ITO glass electrode", Thin Solid Films, vol. 519, pp. 1128- 1134, 2010.
[12] S. Behera et al.," Mercaptoethylpyrazine promoted electrochemistry of redox protein and amperometric biosensing of uric acid", Biosens. Bioelectron. Vol. 23, pp. 556-561 (2007).