@article{(Open Science Index):https://publications.waset.org/pdf/12940, title = {Application of Genetic Engineering for Chromium Removal from Industrial Wastewater}, author = {N. K. Srivastava and M. K. Jha and I. D. Mall and Davinder Singh}, country = {}, institution = {}, abstract = {The treatment of the industrial wastewater can be particularly difficult in the presence of toxic compounds. Excessive concentration of Chromium in soluble form is toxic to a wide variety of living organisms. Biological removal of heavy metals using natural and genetically engineered microorganisms has aroused great interest because of its lower impact on the environment. Ralston metallidurans, formerly known as Alcaligenes eutrophus is a LProteobacterium colonizing industrial wastewater with a high content of heavy metals. Tris-buffered mineral salt medium was used for growing Alcaligenes eutrophus AE104 (pEBZ141). The cells were cultivated for 18 h at 30 oC in Tris-buffered mineral salt medium containing 3 mM disodium sulphate and 46 mM sodium gluconate as the carbon source. The cells were harvested by centrifugation, washed, and suspended in 10 mM Tris HCl, pH 7.0, containing 46 mM sodium gluconate, and 5 mM Chromium. Interaction among induction of chr resistance determinant, and chromate reduction have been demonstrated. Results of this study show that the above bacteria can be very useful for bioremediation of chromium from industrial wastewater.}, journal = {International Journal of Environmental and Ecological Engineering}, volume = {4}, number = {12}, year = {2010}, pages = {633 - 638}, ee = {https://publications.waset.org/pdf/12940}, url = {https://publications.waset.org/vol/48}, bibsource = {https://publications.waset.org/}, issn = {eISSN: 1307-6892}, publisher = {World Academy of Science, Engineering and Technology}, index = {Open Science Index 48, 2010}, }