Qingyue Wang and Jun Morita and Shinichi Nakamura and Di Wu and Xiumin Gong and Miho Suzuki and Makoto Miwa and Daisuke Nakajima
Field Investigation on Modification of Japanese Cedar Pollen Allergen in Urban AirPolluted Area
426 - 431
2010
4
9
International Journal of Environmental and Ecological Engineering
https://publications.waset.org/pdf/12192
https://publications.waset.org/vol/45
World Academy of Science, Engineering and Technology
Cry j 1 is a causative substance of Japanese cedar
pollinosis, and it may deteriorate by Cry j 1 invasion to a lower
respiratory tract. We observed airborne particles containing Cry j 1 by
an immunofluorescence technique using a fluorescence microscope,
and we clarified that Cry j 1 exist as aggregates of airborne fine
particles (< 1.1 μm) in the urban atmosphere. Airborne Cry j 1 may
react with air pollutants and be denature to a substance deteriorated
Japanese cedar pollinosis. Therefore, we applied a sodium dodecyl
sulfatepolyacrylamide gel electrophoresis (SDSPAGE) to evaluate a
Cry j 1 reacted with various air pollutants by liquid phase reaction,
and calculated kinetics constants of Cry j 1 extracted from pollens
collected in various sites and airborne fine particles containing Cry j
1 by using a surface plasmon resonance (SPR) method. As a result, it
is suggested that Cry j 1 may be denatured by air pollutants during
the transportation to the urban atmosphere.
Open Science Index 45, 2010