%0 Journal Article %A Qingyue Wang and Jun Morita and Shinichi Nakamura and Di Wu and Xiumin Gong and Miho Suzuki and Makoto Miwa and Daisuke Nakajima %D 2010 %J International Journal of Environmental and Ecological Engineering %B World Academy of Science, Engineering and Technology %I Open Science Index 45, 2010 %T Field Investigation on Modification of Japanese Cedar Pollen Allergen in Urban Air-Polluted Area %U https://publications.waset.org/pdf/12192 %V 45 %X Cry j 1 is a causative substance of Japanese cedar pollinosis, and it may deteriorate by Cry j 1 invasion to a lower respiratory tract. We observed airborne particles containing Cry j 1 by an immunofluorescence technique using a fluorescence microscope, and we clarified that Cry j 1 exist as aggregates of airborne fine particles (< 1.1 μm) in the urban atmosphere. Airborne Cry j 1 may react with air pollutants and be denature to a substance deteriorated Japanese cedar pollinosis. Therefore, we applied a sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) to evaluate a Cry j 1 reacted with various air pollutants by liquid phase reaction, and calculated kinetics constants of Cry j 1 extracted from pollens collected in various sites and airborne fine particles containing Cry j 1 by using a surface plasmon resonance (SPR) method. As a result, it is suggested that Cry j 1 may be denatured by air pollutants during the transportation to the urban atmosphere. %P 426 - 431