WASET
	%0 Journal Article
	%A Qingyue Wang and  Jun Morita and  Shinichi Nakamura and  Di Wu and  Xiumin Gong and  Miho Suzuki and  Makoto Miwa and  Daisuke Nakajima
	%D 2010
	%J International Journal of Environmental and Ecological Engineering
	%B World Academy of Science, Engineering and Technology
	%I Open Science Index 45, 2010
	%T Field Investigation on Modification of Japanese Cedar Pollen Allergen in Urban Air-Polluted Area
	%U https://publications.waset.org/pdf/12192
	%V 45
	%X Cry j 1 is a causative substance of Japanese cedar
pollinosis, and it may deteriorate by Cry j 1 invasion to a lower
respiratory tract. We observed airborne particles containing Cry j 1 by
an immunofluorescence technique using a fluorescence microscope,
and we clarified that Cry j 1 exist as aggregates of airborne fine
particles (< 1.1 μm) in the urban atmosphere. Airborne Cry j 1 may
react with air pollutants and be denature to a substance deteriorated
Japanese cedar pollinosis. Therefore, we applied a sodium dodecyl
sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) to evaluate a
Cry j 1 reacted with various air pollutants by liquid phase reaction,
and calculated kinetics constants of Cry j 1 extracted from pollens
collected in various sites and airborne fine particles containing Cry j
1 by using a surface plasmon resonance (SPR) method. As a result, it
is suggested that Cry j 1 may be denatured by air pollutants during
the transportation to the urban atmosphere.
	%P 426 - 431