Carbon Sources Utilization Profiles of Thermophilic Phytase Producing Bacteria Isolated from Hot-spring in Malaysia
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Carbon Sources Utilization Profiles of Thermophilic Phytase Producing Bacteria Isolated from Hot-spring in Malaysia

Authors: Noor Muzamil Mohamad, Abdul Manaf Ali, Hamzah Mohd Salleh

Abstract:

Phytases (myo-inositol hexakisphosphate phosphohydrolases; EC 3.1.3.8) catalyze the hydrolysis of phytic acid (myoinositol hexakisphosphate) to the mono-, di-, tri-, tetra-, and pentaphosphates of myo-inositol and inorganic phosphate. Therrmophilic bacteria isolated from water sampled from hot spring. About 120 isolates of bacteria were successfully isolated form hot spring water sample and tested for extracellular phytase producing. After 5 passages of the screening on the PSM media, 4 isolates were found stable in producing phytase enzyme. The 16s RDNA sequencing for identification of bacteria using molecular technique revealed that all isolates those positive in phytase producing are belong to Geobacillus spp. And Anoxybacillus spp. Anoxybacillus rupiensis UniSZA-7 were identified for their carbon source utilization using Phenotype Microarray Plate of Biolog and found they utilize several kind of carbon source provided.

Keywords: Phytase, Phytic Acid, Thermophilic Bacteria, PSM Media and Phytase Assay

Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1078297

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References:


[1] A. S. M. Husein, A-E. Farouk, H. M.Salleh and A. M. Ali. "The potential of Phytate degrading Enzyme for Biotechnological Application: A review," J. Agrobiotech. Food Science, 2009, l1 : 1-13.
[2] A. Pandey et al., "Production, Purification and Properties of Microbial Phytases," Bioresource Technology, 2001, 77 : 203-214.
[3] A. S. Ibrahim and A.I El-diwany. "Isolation and Identification of New Cellulases Producing Thermophilic Bacteria from a Egyptian Hot Spring and Some Properties of the Enzyme," Australian Journal of Basic nad Applied Sciences, 2007, 1: 473-478.
[4] J. Y. H. Tang, J. Carlson, F. M. Ghazali, A. A. Saleha, M. Nishibuchi, Y. Nakaguchi and S. Radu. "Phenotypic MicroArray (PM) profiles (carbon sources and sensitivity to osmolytes and pH) of Campylobacter jejuni ATCC 33560 in response to temperature," International Food Research Journal, 2010, 17: 837-844.
[5] S. J. Yoon., et al. "Isolation and Identification of Phytate-Producing Bacterium, Enterobacter sp., and Enzymatic Properties of Phytase Enzyme," Enzyme and Microbial Technology, 1996, 18:449-454.
[6] R. Devereux and S. S. Wilkinson. "Amplification of ribosomal RNA sequences," in Molecular Microbial Ecology Manual, 2nd ed., 2004, 3.01: 509-522.
[7] Anna Derekova, Carsten Sj├©holm, Rossica Mandeva and Margarita Kambourova. "Anoxybacillus rupiensis sp. Nov., a novel thermophilic bacterium isolated from Rupi basin (Bulgaria)," Extremophiles, 2007, 11:577-583.
[8] R. N. Z. R. A. Rahman, T. C. Leow, A. B. Salleh and M. Basri. "Geobacillus zalihae sp. Nov., a Thermophilic Lipolytic Bacterium Isolated from Palm Oil Mill Effluent in Malaysia," BMC Microbiology, 2007, 7:77.