%0 Journal Article
	%A S. Banerjee and  A. Apte Deshpande and  N. Mandi and  S. Padmanabhan
	%D 2009
	%J International Journal of Bioengineering and Life Sciences
	%B World Academy of Science, Engineering and Technology
	%I Open Science Index 27, 2009
	%T A Novel Cytokine Derived Fusion Tag for Over- Expression of Heterologous Proteins in E. coli
	%U https://publications.waset.org/pdf/11943
	%V 27
	%X We report a novel fusion tag for expressing
recombinant proteins in E. coli. The fusion tag is the C-terminus part
of the human GMCSF gene comprising 45 amino acids, which aid in
over expression of otherwise non expressible genes. Expression of
hIFN a2b with this fusion tag also escapes the requirement of rare
codons for expression. This is also a first report of a small fusion tag
of human origin having affinity to heparin sepharose column
facilitating the purification of fusion protein.
	%P 172 - 176